Evaluation of the anti-inflammatory and analgesic activities of Liu-Shen-Wan and its individual fractions.

Abstract

Liu-Shen-Wan (LSW), a famous traditional Chinese medicine for treatment of upper respiratory tract inflammation, was evaluated for its anti-inflammatory and analgesic activities. Acetic acid-elevated vascular permeability, carboxymethylcellulose sodium (CMC-Na)-induced leukocyte migration and ear edema induced by picryl chloride were used to test anti-inflammatory activity. Moreover, acetic acid-induced writhing and hot-plate tests were used to determine analgesic effect. It was observed that LSW exerted significant anti-inflammatory and analgesic activities in these models at doses of 30 and 90mg/kg crude drug in vivo. In addition, LSW potently inhibited proliferation of human peripheral blood mononuclear cell (PBMC) stimulated by streptococcal pyrogenic exotoxin at doses of 0.5-5microg/ml in vitro. LSW was then partitioned with chloroform, methanol, water and mineral fraction. Several fractions inhibited inflammation and pain in varying degrees. Among them, chloroform fraction was the most active in hot-plate and writhing tests, and exerted the remarkable inhibitory effect on human PBMC proliferation. Methanol and water fractions had more suppressive activities in vascular permeability, leukocyte migration and PC-DTH tests. These results suggest that LSW has significantly anti-inflammatory and analgesic activities. The chloroform fraction is a key fraction of LSW to the overall anti-inflammatory and analgesic effects, while methanol and water fractions also partly contribute to anti-inflammatory activities of LSW.

• Authors: Hong-Yue Ma, Jun-Ping Kou, Jing-Rong Wang, Bo-Yang Yu
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 112 Issue 1 Pg. 108-14 (May 30 2007) ISSN: 0378-8741 [Print] Ireland
• PMID: 17368990 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics
• Anti-Inflammatory Agents
• Complex Mixtures
• Liu-Shen-Wan

Topics

• Analgesics (chemistry, pharmacology)
• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Capillary Permeability (drug effects)
• Chemical Fractionation
• Chemotaxis, Leukocyte (drug effects)
• Complex Mixtures (chemistry, pharmacology)
• Disease Models, Animal
• Female
• Humans
• Hypersensitivity, Delayed (prevention & control)
• In Vitro Techniques
• Leukocytes, Mononuclear (drug effects, physiology)
• Male
• Medicine, Chinese Traditional
• Mice
• Mice, Inbred ICR