Enhanced expression of the nuclear envelope LAP2 transcriptional repressors in normal and malignant activated lymphocytes.

Abstract

Extensive research in recent years has broadened the functions of nuclear envelope proteins beyond simply stabilizing the nucleus architecture. Particularly, integral nuclear membrane proteins, such as the alternative spliced isoforms of lamina-associated polypeptide 2 (LAP2), have been shown to be important for the initiation of replication and repression of transcription. The latter is regulated by epigenetic changes, induced by the binding of LAP2beta to histone deacetylase-3 (HDAC3), resulting in histone H4 deacetylation. Involvement of nuclear envelope proteins in pathological proliferative conditions, mainly those involving abnormal recruitment and activation of HDACs, is still unknown. In this paper, we show that various nuclear envelope proteins are highly expressed in normal and malignant activated lymphocytes. Specifically, rapidly replicating cells of various hematological malignancies highly express LAP2beta, while slowly proliferating malignant cells of chronic malignant hematological diseases do not. Taking together the elevated expression of LAP2beta in highly proliferative malignant cells with its known ability to modify histones through binding with HDAC3 raises the possibility of its role in hematological malignancies involving aberrant activity of HDAC3. Based on our presented results, we believe that the LAP2-HDAC regulatory pathway should be studied as a new target for rational therapy.

• Authors: Raz Somech, Einav Nili Gal-Yam, Sigal Shaklai, Orit Geller, Ninette Amariglio, Gideon Rechavi, Amos J Simon
• Journal: Annals of hematology (Ann Hematol) Vol. 86 Issue 6 Pg. 393-401 (Jun 2007) ISSN: 0939-5555 [Print] Germany
• PMID: 17364180 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Membrane Proteins
• lamina-associated polypeptide 2
• Histone Deacetylases
• histone deacetylase 3

Topics

• DNA-Binding Proteins (metabolism)
• Hematologic Neoplasms (metabolism)
• Histone Deacetylases (metabolism)
• Humans
• Lymphocytes (metabolism)
• Lymphoproliferative Disorders (blood, physiopathology)
• Membrane Proteins (metabolism)
• Nuclear Envelope (metabolism)
• Signal Transduction (physiology)
• Transcription, Genetic (physiology)
• Up-Regulation