Abstract |
Here, we conduct dose-response studies in mice for the elicitation of CD8 T cells by a DNA vaccine that expresses HIV Gag. For DNA doses ranging from 1 to 100 microg, the studies revealed greater than 10-fold increases in anti-Gag CD8 T cells following a DNA prime or a DNA prime and a constant modified vaccinia Ankara (MVA) boost. These results are in contrast to dose-response studies for MVA vectors expressing Gag, where only 2-3-fold increases in anti-Gag CD8 T cells were elicited by 100-fold increases in dose.
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Authors | Jinyan Liu, Michael Hellerstein, Michael McDonnel, Rama Rao Amara, Linda S Wyatt, Bernard Moss, Harriet L Robinson |
Journal | Vaccine
(Vaccine)
Vol. 25
Issue 15
Pg. 2951-8
(Apr 12 2007)
ISSN: 0264-410X [Print] Netherlands |
PMID | 17360078
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- HIV Core Protein p24
- Vaccines, DNA
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Topics |
- Animals
- CD8-Positive T-Lymphocytes
(immunology)
- Dose-Response Relationship, Immunologic
- Female
- HIV Core Protein p24
(genetics, immunology)
- Mice
- Mice, Inbred BALB C
- Vaccines, DNA
(administration & dosage, genetics, immunology)
- Vaccinia virus
(immunology)
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