Dose-response studies for the elicitation of CD8 T cells by a DNA vaccine, used alone or as the prime for a modified vaccinia Ankara boost.

Here, we conduct dose-response studies in mice for the elicitation of CD8 T cells by a DNA vaccine that expresses HIV Gag. For DNA doses ranging from 1 to 100 microg, the studies revealed greater than 10-fold increases in anti-Gag CD8 T cells following a DNA prime or a DNA prime and a constant modified vaccinia Ankara (MVA) boost. These results are in contrast to dose-response studies for MVA vectors expressing Gag, where only 2-3-fold increases in anti-Gag CD8 T cells were elicited by 100-fold increases in dose.
AuthorsJinyan Liu, Michael Hellerstein, Michael McDonnel, Rama Rao Amara, Linda S Wyatt, Bernard Moss, Harriet L Robinson
JournalVaccine (Vaccine) Vol. 25 Issue 15 Pg. 2951-8 (Apr 12 2007) ISSN: 0264-410X [Print] Netherlands
PMID17360078 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • HIV Core Protein p24
  • Vaccines, DNA
  • Animals
  • CD8-Positive T-Lymphocytes (immunology)
  • Dose-Response Relationship, Immunologic
  • Female
  • HIV Core Protein p24 (genetics, immunology)
  • Mice
  • Mice, Inbred BALB C
  • Vaccines, DNA (administration & dosage, genetics, immunology)
  • Vaccinia virus (immunology)

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