Preservation of the pHi during ischemia via PKC by intermittent hypoxia.

Abstract

In intermittent hypoxia adaptation (IHA) rat cardiomyocytes, the relationship between activated protein kinase C and intracellular acidification regulation during ischemia-reperfusion (I/R) was tested. Using [H(+)] indicator BCECF-AM, we analyzed the alterations of intracellular pH (pH(i)) in normoxia and IHA rat cardiomyocytes during I/R. With the time of ischemia, the pH(i) decreased progressively in normal cardiomyocytes, but fewer alterations in IHA myocytes. Treatment of IHA and normoxia cardiomyocytes with 5 microM chelerythrine delayed the pH(i) recovery during post-ischemia. In contrast, the application of 1 microM phorbol 12-myristate 13-acetate in normoxia cardiomyocytes preserved the pH(i) during I/R, which was similar to that in IHA cardiomyocytes. Our data suggest that the stable PKC activation might contribute to preservation of the pH(i), which may be beneficial to maintain cardiac function during I/R in IHA rats.

• Authors: Jun Li, Hao Zhang, Wei-Zhong Zhu, Zhuo Yu, Ang Guo, Huang-Tian Yang, Zhao-Nian Zhou
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 356 Issue 2 Pg. 329-33 (May 04 2007) ISSN: 0006-291X [Print] United States
• PMID: 17359938 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Kinase C

Topics

• Adaptation, Physiological
• Animals
• Disease Models, Animal
• Hydrogen-Ion Concentration
• Hypoxia (enzymology, physiopathology)
• Ischemia (physiopathology)
• Male
• Protein Kinase C (physiology)
• Rats
• Rats, Sprague-Dawley
• Reperfusion