Two independent apolipoprotein A5 haplotypes modulate postprandial lipoprotein metabolism in a healthy Caucasian population.

Abstract

BACKGROUND:

Apolipoprotein A5 (APOA5) plays an important role in plasma triacylglycerol (TG) homeostasis. Five polymorphisms (1131T>C, c.-3A>G, c.56C>G, IVS3+476G>A, and c.1259T>C) in the APOA5 gene define three common haplotypes (APOA5*1, APOA5*2, and APOA5*3) in Caucasian individuals. Our aim was to determine whether these haplotypes could modulate the postprandial response in young healthy males.

DESIGN AND METHODS:

Eighty-eight APO E3/3 volunteers [67 with (-1131T and 56C) APOA5*1 haplotype, 12 with (-1131C and 56C) APOA5*2 haplotype, and nine with (-1131T and 56G) APOA5*3 haplotype] underwent a fat load test consisting of the consumption of 1 g of fat per kilogram body weight and 60,000 IU vitamin A. Blood samples were taken at time 0, at every hour until the sixth hour, and at every 2.5 h until the 11th hour. Total plasma cholesterol (C) and TG, and C, TG, apolipoprotein B-100, apolipoprotein B-48, and retinyl palmitate in lipoprotein fractions were determined.

RESULTS:

Subjects with the APOA5*2 and APOA5*3 haplotypes had a higher area under the curve of total plasma TG (P = 0.03), large TG-rich lipoprotein (TRL)-TG (P = 0.02), small TRL-TG (P = 0.04), small TRL-C (P = 0.04), large TRL-C (P = 0.03), and small apolipoprotein B100 (P = 0.04) than subjects with the APOA5*1 haplotype.

CONCLUSIONS:

Our findings show that the presence of the APOA5*2 and APOA5*3 haplotypes in the APOA5 gene is associated with a higher postprandial response that could be involved in the higher risk of coronary heart disease associated with the 56G and -1131C alleles.

• Authors: Rafael Moreno-Luna, Francisco Perez-Jimenez, Carmen Marin, Pablo Perez-Martinez, Purificacion Gomez, Yolanda Jimenez-Gomez, Javier Delgado-Lista, Juan A Moreno, Toshiko Tanaka, Jose M Ordovas, Jose Lopez-Miranda
• Journal: The Journal of clinical endocrinology and metabolism (J Clin Endocrinol Metab) Vol. 92 Issue 6 Pg. 2280-5 (Jun 2007) ISSN: 0021-972X [Print] United States
• PMID: 17356049 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• APOA5 protein, human
• Apolipoprotein A-V
• Apolipoprotein B-100
• Apolipoprotein B-48
• Apolipoproteins A
• Lipoproteins
• Triglycerides

Topics

• Adult
• Apolipoprotein A-V
• Apolipoprotein B-100 (genetics, metabolism)
• Apolipoprotein B-48 (genetics, metabolism)
• Apolipoproteins A (genetics, metabolism)
• Coronary Disease (ethnology, genetics)
• Energy Metabolism (genetics)
• Genetic Predisposition to Disease
• Haplotypes
• Humans
• Lipoproteins (metabolism)
• Male
• Polymorphism, Single Nucleotide
• Postprandial Period (physiology)
• Risk Factors
• Triglycerides (blood)
• White People (genetics)