Abstract | BACKGROUND & OBJECTIVE: METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify 24 genes of TCRBV subfamily from peripheral blood lymphocytes of 9 leukemia patients after haploidentical BMT, and 5 healthy donors as control. The PCR products were analyzed by GeneScan to evaluate the clonality of BV subfamily, characteristics of complementarity determining region 3 (CDR3), and usage rate of BV subfamily. Graft-versus-host disease (GVHD)-related monoclonal bands were sequenced. RESULTS: During 10-19 months after haploidentical BMT, the usage of TCRBV subfamily was still restricted. Deletion of some BV subfamily members was detected, while others expanded in monoclonal or oligoclonal. For 4 patients with stable disease, the expression of 9-14 BV subfamily members was detected, and more than 50% of them were polyclones. For other 5 patients with GVHD or cytomegalovirus (CMV)-pp65 infection, the usage of TCRBV decreased obviously(P<0.05), the expression of CDR3 were monoclonal or oligoclonal, only 30% were polyclonal. No common monoclonal BV subfamily members were detected. After treatment, the usage of BV subfamily and CDR3 polymorphism were increased in 2 patients. By analyzing the sequences associated with GVHD, none of the clones appeared to share any similarity in amino acid motif. CONCLUSIONS: In 10-19 months after haploidentical BMT, the usage of TCRBV subfamily members is still restricted. In stable condition, there are 9-14 BV subfamily members expressed and dominated by polyclones. In active condition, the expression of BV subfamily members is decreased and dominated by monoclones or oligoclones. A group of CDR3 molecules related to GVHD show no common amino acid motif to be shared.
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Authors | Yue-Wen Fu, De-Pei Wu, Yu-Feng Feng, Wei-Rong Chang, Zi-Ling Zhu, Ping Zhu |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 26
Issue 3
Pg. 236-40
(Mar 2007)
China |
PMID | 17355783
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Complementarity Determining Regions
- Receptors, Antigen, T-Cell, alpha-beta
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Topics |
- Adolescent
- Adult
- Amino Acid Sequence
- Bone Marrow Transplantation
(adverse effects)
- Child
- Clone Cells
- Complementarity Determining Regions
(genetics, metabolism)
- Cytomegalovirus Infections
- Female
- Graft vs Host Disease
(etiology, immunology, pathology)
- Humans
- Leukemia, Myeloid, Acute
(surgery, virology)
- Male
- Molecular Sequence Data
- Polymorphism, Genetic
- Receptors, Antigen, T-Cell, alpha-beta
(genetics, metabolism)
- T-Lymphocytes
(immunology, metabolism, pathology)
- Young Adult
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