Abstract | OBJECTIVE: METHODS: Diabetic rats with back full-thickness skin lesion and syndrome of blood stasis and qi deficiency were divided in to five groups: untreated group, basic fibroblast growth factor (bFGF)-treated group, Yiqi Huayu Recipe (a recipe for replenishing qi and resolving stagnation)-treated group, Yiqi Recipe (a recipe for replenishing qi)-treated group and Huayu Recipe (a recipe for resolving stagnation)-treated group, and another eight normal rats served as normal control group. Immunohistochemical method and image analysis were used to test the expressions of HIF-1alpha and VEGF in granulation tissue of skin ulcers in rats with diabetes. RESULTS: In the untreated group, the expression of HIF-1alpha was significantly increased and the expression of VEGF was significantly decreased as compared with those in the normal control group (P<0.01). The expression of HIF-1alpha was obviously lower (P<0.01) and the expression of VEGF was significantly higher in the four drug treated groups as compared with those in the untreated group. In the Yiqi Huayu Recipe-treated group, the expression of HIF-1alpha was obviously lower than those in the bFGF-treated, Yiqi Recipe-treated and Huayu Recipe-treated groups (P<0.05 or P<0.01), and the expression of VEGF was significantly higher than that in the bFGF-treated group (P<0.01). CONCLUSION: Chinese herbs for replenishing qi and resolving stagnation can promote the wound healing in rats through reducing the expression of HIF-1alpha, accelerating the expression of VEGF in granulation tissue of skin ulcers in rats with diabetes and ameliorating the status of ischemia and hypoxia.
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Authors | Hua-fa Que, Yuan-yin Zhu, Yun-fei Wang, Zhen Zhang, Jie-nan Xu, Jie Xing, Han-jun Tang |
Journal | Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
(Zhong Xi Yi Jie He Xue Bao)
Vol. 5
Issue 2
Pg. 165-9
(Mar 2007)
ISSN: 1672-1977 [Print] China |
PMID | 17352873
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- Hypoxia-Inducible Factor 1, alpha Subunit
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Diabetes Mellitus, Experimental
(complications, metabolism)
- Drugs, Chinese Herbal
(pharmacology)
- Granulation Tissue
(metabolism, pathology)
- Hypoxia-Inducible Factor 1, alpha Subunit
(biosynthesis)
- Male
- Qi
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Skin Ulcer
(etiology, metabolism)
- Vascular Endothelial Growth Factor A
(biosynthesis)
- Wound Healing
(drug effects)
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