Abstract | BACKGROUND: Previous studies have shown that, cultured rat pancreatic carcinoma cells, derived from azaserine-induced acinar tumours, yield tumours with a ductal phenotype. MATERIALS AND METHODS: RESULTS: Immunoreactivity for p53 protein was found in 86% of intrapancreatic tumours and in 100% of subcutaneous tumours. The average fraction of positive carcinoma cells was over 50%. Normal rat pancreas showed only slight positive or negative staining for p53. Bcl-2 did not show positive immunoreactivity in rat tumour samples. For PCNA all tumour samples showed positive staining. Also normal pancreas of 6-week-old animals were clearly positive, whereas the samples of the older animals were only slightly positive. CONCLUSION: Possible mutations in the p53 tumour suppressor gene and a strong expression of PCNA were shown in carcinoma cell line-induced rat pancreatic tumours. These features of the rat pancreatic tumour model resemble human pancreatic carcinoma and may favour the use of this model in pancreatic cancer studies.
|
Authors | Kimmo Mäkinen, Sami Loimas, Tapio Hakala, Matti Eskelinen |
Journal | Anticancer research
(Anticancer Res)
2007 Jan-Feb
Vol. 27
Issue 1A
Pg. 23-6
ISSN: 0250-7005 [Print] Greece |
PMID | 17352211
(Publication Type: Journal Article)
|
Chemical References |
- Proliferating Cell Nuclear Antigen
- Proto-Oncogene Proteins c-bcl-2
- Tumor Suppressor Protein p53
|
Topics |
- Animals
- Disease Models, Animal
- Immunohistochemistry
- Male
- Pancreatic Neoplasms
(metabolism)
- Proliferating Cell Nuclear Antigen
(biosynthesis)
- Proto-Oncogene Proteins c-bcl-2
(biosynthesis)
- Rats
- Rats, Inbred Lew
- Tumor Suppressor Protein p53
(biosynthesis)
|