Abstract | OBJECTIVE: METHODS: The diagnosis was based on clinical, biochemical and radiological data. The subjects were composed of three groups, the patient group had 30 children, the vitamin D deficiency group 35 children, and the control group 60 normal children. The VDRSC genotypes of the three groups were determined by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. RESULTS: There was significant difference in the frequencies distribution of VDRSC genotypes (chi(2) = 13.184, P = 0.010) and VDRSC alleles (chi(2) = 8.975, P = 0.011) among the three groups; the frequency of the FF genotype (56.7%) in the patient group was significantly higher than that in the control group (21.7%, P = 0.006) and that in the vitamin D deficiency group (22.9%, P = 0.002). The frequency of the F alleles in the patient group (70.0%) was significantly higher than that in the control group (48.3%, P = 0.006) and that in the vitamin D deficiency group (47.1%, P = 0.009). Multiple logistic regression analysis showed that FF genotype had a higher risk of delayed rickets (OR = 3.120), indicating that FF genotype may be significantly associated with delayed rickets. CONCLUSION: There is the possibility that the VDRSC polymorphism might be important in determining the hereditary susceptibility of children to development of delayed rickets.
|
Authors | Juan-juan Lu, Yu-ning Li, Yu Jin, Lei Li |
Journal | Zhonghua er ke za zhi = Chinese journal of pediatrics
(Zhonghua Er Ke Za Zhi)
Vol. 45
Issue 1
Pg. 46-9
(Jan 2007)
ISSN: 0578-1310 [Print] China |
PMID | 17349151
(Publication Type: English Abstract, Journal Article)
|
Chemical References |
- Codon, Initiator
- Receptors, Calcitriol
|
Topics |
- Case-Control Studies
- Child
- Codon, Initiator
- Female
- Gene Frequency
- Genetic Predisposition to Disease
- Genotype
- Humans
- Logistic Models
- Male
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Receptors, Calcitriol
(genetics)
- Rickets
(genetics)
- Vitamin D Deficiency
(genetics)
|