Abstract | BACKGROUND/AIMS: METHODS: A unilateral ureteral obstruction (UUO) model was employed, and sham-operated rats were used as controls. Renal lesions and the expression of Arkadia, Smurf2, Smad7, TbetaRI, TGF-beta1, and type 1 collagen (COL-1) were detected by Western blot, immunoprecipitation, immunohistochemistry, and/or reverse transcription-polymerase chain reaction. RESULTS: CONCLUSION: Reduction of Smad7 resulting from ubiquitin-dependent degradation may be mainly attributed to Arkadia, and Arkadia-Smad7-mediated positive regulation of TGF-beta signaling may play a promoting role in the progression of tubulointerstitial fibrosis.
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Authors | Fu-You Liu, Xiao-Zhao Li, You-Ming Peng, Hong Liu, Ying-Hong Liu |
Journal | American journal of nephrology
(Am J Nephrol)
Vol. 27
Issue 2
Pg. 176-83
( 2007)
ISSN: 1421-9670 [Electronic] Switzerland |
PMID | 17347560
(Publication Type: Journal Article)
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Copyright | Copyright 2007 S. Karger AG, Basel. |
Chemical References |
- Receptors, Transforming Growth Factor beta
- Smad7 Protein
- Transforming Growth Factor beta
- Ubiquitin-Protein Ligases
- Protein Serine-Threonine Kinases
- Activin Receptors, Type I
- Receptor, Transforming Growth Factor-beta Type I
- Tgfbr1 protein, rat
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Topics |
- Activin Receptors, Type I
(biosynthesis)
- Animals
- Disease Models, Animal
- Fibrosis
- Kidney Diseases
(etiology, pathology, physiopathology)
- Male
- Protein Serine-Threonine Kinases
- Rats
- Rats, Sprague-Dawley
- Receptor, Transforming Growth Factor-beta Type I
- Receptors, Transforming Growth Factor beta
(biosynthesis)
- Signal Transduction
- Smad7 Protein
(biosynthesis)
- Transforming Growth Factor beta
(metabolism)
- Ubiquitin-Protein Ligases
(biosynthesis)
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