Abstract | OBJECTIVE: METHODS: FAS mRNA expression in human MM cell line U266, RPMI8226 cell was assayed by RT-PCR. The proliferation inhibition rate of U266 cells was assayed by MTr analysis. Cell apoptosis and cycle distribution were evaluated by flow cytometry (FCM). RESULTS: FAS mRNA was highly expressed in human multiple myeloma cell lines as compared with healthy donor PBMNCs. After U266 cells were treated with cerulenin (the concentrations from 5 microg/ml to 640 microg/ ml) for 24 h, the cell proliferation was markedly inhibited with a dose related manner, while the inhibition rate of human skin fibroblast cells were all lower than 30%. When U266 cells were treated with 20 pjg/ml cerulenin for 12 h and 24 h, the early apoptosis rate revealed by Annexin V/PI were 56. 9% and 69. 3% respectively, being higher than that of the blank controls (4. 3% and 1.8%, P < 0. 01). Cell cycle analysis showed it was blocked in S phase. Conclusion FAS is highly expressed in human MM. Cerulenin could induce apoptosis and inhibit proliferation of U266 cells. FAS might be a new potential target for multiple myeloma treatment.
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Authors | Wei-qin Wang, Xiao-ying Zhao, Geng-bo Xu, Yun Liang |
Journal | Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
(Zhonghua Xue Ye Xue Za Zhi)
Vol. 27
Issue 10
Pg. 675-7
(Oct 2006)
ISSN: 0253-2727 [Print] China |
PMID | 17343199
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cerulenin
- Fatty Acid Synthases
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Topics |
- Apoptosis
(drug effects)
- Cell Proliferation
(drug effects)
- Cerulenin
(pharmacology)
- Dose-Response Relationship, Drug
- Fatty Acid Synthases
(antagonists & inhibitors, biosynthesis)
- Humans
- Multiple Myeloma
(metabolism, pathology)
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Cells, Cultured
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