Abstract | OBJECTIVE: METHODS: The mRNA expressions of collagens, MMP-13 and TIMP-1 were detected by RT-PCR. The strips were scanned by gel imaging system scanner, and the optical density was autocalculated by computer. RESULTS: Compared with those of the sham-operative group, the mRNA expressions of collagen I , Ill and X and MMP-13 of the model rats increased markedly (P < 0.01), which were lowered by YHBR and its disassembled recipes (P < 0.01 or P < 0.05), and the levels after YHBR treatment were significantly different to those after Western medicine treatment. However, no remarkable change was found in TIMP-1 mRNA expression in the model rats (P > 0.05). CONCLUSION: In the degenerated intervertebral disc the mRNA expressions of collagen I , III, X and MMP-13 increased, TIMP-1 mRNA expression decreased and the proportion of MMPs/TIMP was in imbalance. YHBR and its disassembled recipes could postpone the degeneration of intervertebral disc through regulating mRNA expressions of collagens and their correlated metabolic enzymes.
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Authors | Qi Shi, Yong-Jun Wang, Chen-Guang Li |
Journal | Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
(Zhongguo Zhong Xi Yi Jie He Za Zhi)
Vol. 27
Issue 2
Pg. 142-6
(Feb 2007)
ISSN: 1003-5370 [Print] China |
PMID | 17343002
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- RNA, Messenger
- Tissue Inhibitor of Metalloproteinase-1
- yiqi huayu bushen
- Collagen
- Matrix Metalloproteinase 13
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Topics |
- Animals
- Cervical Vertebrae
- Collagen
(biosynthesis, genetics)
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Extracellular Matrix
(drug effects, metabolism)
- Intervertebral Disc
(drug effects, metabolism, pathology)
- Intervertebral Disc Displacement
(drug therapy, genetics, metabolism)
- Male
- Matrix Metalloproteinase 13
(biosynthesis, genetics)
- Phytotherapy
- RNA, Messenger
(biosynthesis, genetics)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Inhibitor of Metalloproteinase-1
(biosynthesis, genetics)
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