Cellular localization, binding sites, and pharmacologic effects of TFF3 in experimental colitis in mice.

Abstract	Trefoil factors (TFFs) are essential for protection and restitution of the gastrointestinal mucosa but many aspects of TFF biology are unclear. Our aim was to compare the localization of endogenous TFFs and binding sites for injected TFF3 in the colon of healthy and colitic mice and to study the effect of TFF3 on dextrane sulfate sodium (DSS)-induced colitis in mice. Expression of endogenous TFF1-3 was examined by in situ hybridization and immunohistochemistry, and the distribution of intravenously, intraperitoneally, and subcutaneously administered (125)I-TFF3 by autoradiography and gamma-counting. The effect of systemically administered TFF3 on DSS-induced colitis was assessed. We found increased expression of endogenous TFF3 and increased binding of injected (125)I-TFF3 in the colon of animals with DSS-induced colitis. The distribution of intraperitoneally and subcutaneously administered (125)I-TFF3 was comparable. Systemic administration of the peptides reduced the severity of colitis. Expression of endogenous TFF3 and binding of systemically administered TFF3 are increased in DSS-induced colitis. Systemic administration of TFF3 attenuates the disease. These findings suggest a role of TFF3 in mucosal protection.
Authors	Stine Kjellev, Lars Thim, Charles Pyke, Steen S Poulsen
Journal	Digestive diseases and sciences (Dig Dis Sci) Vol. 52 Issue 4 Pg. 1050-9 (Apr 2007) ISSN: 0163-2116 [Print] United States
PMID	17342398 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Iodine Radioisotopes Mucins Peptides TFF3 protein, human Tff3 protein, mouse Trefoil Factor-3 Dextran Sulfate
Topics	Animals Autoradiography Binding Sites Colitis (chemically induced, metabolism, pathology) Colon (metabolism, pathology) Dextran Sulfate Female Goblet Cells Immunohistochemistry Injections, Intraperitoneal Injections, Intravenous Injections, Subcutaneous Iodine Radioisotopes Mice Mice, Inbred BALB C Mucins (administration & dosage, metabolism, pharmacokinetics, pharmacology) Peptides (pharmacokinetics, pharmacology) Tissue Distribution Trefoil Factor-3

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