[The protective effects of intermedin 1-53 on oleic acid induced acute lung injury in rats].

Abstract

OBJECTIVE:

To investigate the effects of intermedin 1-53 on oleic acid induced acute lung injury (ALI) in rats and its possible mechanisms.

METHODS:

Twenty-four male Wistar rats, weighting 280-300 g, were randomly divided into three groups (n = 8). In the oleic acid group, ALI was induced by injecting oleic acid (0.2 ml/kg) intravenously. In the intervention group, the rats received oleic acid as above, but intraperitoneal injection of intermedin 1-53 (2 nmol/kg in l ml saline) was given 1 h before and 2 h after oleic acid infusion, respectively. The control group was given normal saline (0.2 ml/kg). The animals were observed for 6 h after intravenous infusion. The histopathological changes of lung tissue were studied. The arterial blood gases, lung wet/dry weight ratio (W/D), leukocyte count and neutrophil differentials in bronchoalveolar lavage fluid (BALF) were measured. Contents of intermedin 1-53 in the plasma were detected with radioimmunoassay, myeloperoxidase activity, and malondialdehyde (MDA) and conjugated diene (C-diene) level of lung tissue were also measured.

RESULTS:

The PaO(2) was higher and lung wet/dry weight ratio, leukocyte count and neutrophil counts in BALF were significantly lower in the intermedin group [(81.7 +/- 4.6) mm Hg (1 mm Hg = 0.133 kPa), 5.49 +/- 0.63, (57.9 +/- 7.4) x 10(4)/ml, 0.718 +/- 0.085, respectively] as compared to the oleic acid group [(60.8 +/- 3.2) mm Hg, 6.18 +/- 0.34, (122.0 +/- 16.6) x 10(4)/ml, 0.878 +/- 0.026], q value was 16.74, 3.43, 17.23, 6.32, respectively, all P < 0.05 or 0.01).

CONCLUSIONS:

Intermedin 1 - 53 treatment significantly attenuated oleic acid-induced lung injury in rats. Intermedin 1-53 exerts pulmo-protective effects by inhibiting formation of lipid oxide products.

• Authors: Xiao-min Yu, Xin-min Liu, Yong-fen Qi, Zhi-gang Zhang, Chao-shu Tang
• Journal: Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases (Zhonghua Jie He He Hu Xi Za Zhi) Vol. 29 Issue 12 Pg. 808-11 (Dec 2006) ISSN: 1001-0939 [Print] China
• PMID: 17327082 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Pyrrolizidine Alkaloids
• Oleic Acid
• Malondialdehyde
• Peroxidase
• indicine

Topics

• Acute Lung Injury (chemically induced, metabolism)
• Animals
• Lung (metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Oleic Acid (adverse effects)
• Peroxidase (metabolism)
• Pyrrolizidine Alkaloids (blood, pharmacology)
• Rats
• Rats, Wistar