Interactions of acyl-coenzyme A with phosphatidylcholine bilayers and serum albumin.

Abstract

Interactions of oleoyl- and octanoyl-coenzyme A (CoA) with phosphatidylcholine (PC) vesicles and bovine serum albumin (BSA) were investigated by NMR spectroscopy. Binding of acyl-CoA to small unilamellar PC vesicles and to BSA was detected by changes in 13C and 31P chemical shifts relative to the chemical shifts for aqueous acyl-CoA. When oleoyl-CoA (less than or equal to 15 mol %) was added to preformed vesicles, the 13C thioester signal (200.1 ppm) was upfield from the signal for micellar oleoyl-CoA (201.7 ppm), suggesting decreased H-bonding (partial dehydration) at the carbonyl group upon binding to the bilayer. When vesicles were prepared by cosonication of oleoyl-CoA and PC, a second peak (199.8 ppm) was seen. The major peak at 200.1 ppm broadened and shifted after addition of Dy(NO3)3 and was not seen after addition of BSA, while the peak at 199.8 ppm was unaffected by either perturbation. Thus, oleoyl-CoA in each bilayer leaflet was distinguished, and transbilayer movement was shown to be slow (t 1/2 greater than or equal to hours). PC vesicles remained intact with less than or equal to 15 mol % oleoyl-CoA, while higher oleoyl-CoA proportions produced mixed micelles. In contrast, 13C spectra revealed rapid exchange (ms) of octanoyl-CoA between the aqueous phase and PC vesicles and a low affinity for the bilayer. Thus, the binding affinity of acyl-CoA for PC bilayers is dependent on the acyl chain length. Oleoyl-CoA in the presence of BSA (1 mol/mol) gave rise to three carbonyl signals at 197.2-203.6 ppm. With 2-5 mol of oleoyl-CoA/BSA, 1-2 additional signals were observed. None of the signals corresponded to unbound oleoyl-CoA. Addition of [13C]carboxyl-enriched oleic acid to oleoyl-CoA/BSA mixtures revealed simultaneous binding of oleic acid and oleoyl-CoA to BSA, with some perturbation of binding interactions. Thus, BSA contains multiple binding sites for oleoyl-CoA and can bind fatty acid and acyl-CoA simultaneously.

• Authors: J G Boylan, J A Hamilton
• Journal: Biochemistry (Biochemistry) Vol. 31 Issue 2 Pg. 557-67 (Jan 21 1992) ISSN: 0006-2960 [Print] United States
• PMID: 1731912 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Acyl Coenzyme A
• Lipid Bilayers
• Micelles
• Phosphatidylcholines
• oleoyl-coenzyme A
• Serum Albumin, Bovine

Topics

• Acyl Coenzyme A (chemistry)
• Lipid Bilayers (chemistry)
• Magnetic Resonance Spectroscopy
• Micelles
• Phosphatidylcholines (chemistry)
• Protein Binding
• Serum Albumin, Bovine (chemistry)