Contrast-enhanced computed tomography (CECT) and contrast-enhanced destruction-replenishment subharmonic ultrasound (CEDRSU) were used to quantify blood flow and
tumor viability during antiangiogenic
therapy.
SU11657 or placebo was administered to R3230AC
tumor-baring rats over a two-week period. CEDRSU vascular volume (ASI) and volume flow (VF) and CECT perfusion (PR) and permeability (PM) measurements were made on day 0, 7 and 14. The percent change in imaging parameters was calculated between day 0 and 7 and 14. Serum
drug level (SDL) was compared with imaging parameters. Imaging estimates of
tumor viability were compared with histology images on day 14. The percent change in imaging measures for control and treated groups were significantly different on day 7(ASI, p = 0.02; VF, p = 0.008, PR, p = 0.0007; PM, p = 0.003) and 14 (ASI, p = 0.0004; VF, p = 0.002, PR, p = 0.003; PM, p = 0.005). Imaging identified animals with lower SDLs as having higher
tumor vascularity and flow. Spatial estimates of
tumor viability correlated with histology (CEDRSU, r(2) = 0.92, p << 0.001; CT, r(2) = 0.86, p << 0.001). CEDRSU and CECT provide measures of blood flow and viability in
tumors during antiangiogenic
therapy.
Tumors with higher flow were identified in animals with lower SDL.
SU11657 treatment results in decreased
tumor flow and viability.