Abstract |
Isopentenyladenosine (i(6)A) is a product of isopentenyltransferases and, in mammals, occurs either bound to tRNA or as a free nucleoside. Sporadic reports have suggested an anticancer effect of i(6)A, mostly on leukemia cells. The present analysis of 9 human epithelial cancer cell lines derived from different types of malignant tissue revealed complete suppression of clonogenic activity in 8 of the lines after exposure to i(6)A at a concentration of 10 muM. Mechanistic studies showed that i(6)A tumor suppressor activity is associated with inhibition of cell proliferation, a block in DNA synthesis and morphological changes. These results point to i(6)A and to its possible derivatives as a new potential class of wide-spectrum anticancer agents.
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Authors | Monica Spinola, Francesca Colombo, F Stefania Falvella, Tommaso A Dragani |
Journal | International journal of cancer
(Int J Cancer)
Vol. 120
Issue 12
Pg. 2744-8
(Jun 15 2007)
ISSN: 0020-7136 [Print] United States |
PMID | 17304507
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- DNA, Neoplasm
- Isopentenyladenosine
- Geranyltranstransferase
- Caspase 3
- Caspase 7
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Topics |
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Blotting, Western
- Caspase 3
(metabolism)
- Caspase 7
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Shape
(drug effects)
- DNA, Neoplasm
(biosynthesis)
- Dose-Response Relationship, Drug
- Epithelial Cells
(drug effects, metabolism, pathology)
- Geranyltranstransferase
(metabolism)
- HT29 Cells
- Humans
- Isopentenyladenosine
(pharmacology, therapeutic use)
- Neoplasms
(drug therapy, metabolism, pathology)
- Time Factors
- Tumor Stem Cell Assay
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