Abstract | OBJECTIVE: METHODS: DC vaccine prepared by transfection of adenovirus mediated melanoma-associated antigen gene (gp100) into bone marrow-derived dendritic cell (DC) was used to study the immuno- therapeutic effect and the mechanism of lentinan (LNT) in different dosages, used alone or combined with gp100-DC for treatment of B16 melanoma bearing mice. RESULTS: After being treated with LNT combining gp100-DC, the growth of malignant melanoma was inhibited with the tumor-free survival in the experimental animals being 66.7%. The treatment could also significantly enhance the activity of cytotoxicity T lymphocyte (CTL) and natural killer (NK) cells, elevate the levels of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in splenocytes, and histological examination showed that a large amount of inflammatory cells infiltrated inside and around the tumor, and obvious necrosis of tumor cells was found. CONCLUSION: By combined use with LNT the anti- tumor immuno-reaction of DCV vaccine could be enhanced effectively.
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Authors | Jun Wang, Zhi-dong Zhou, Da-jing Xia |
Journal | Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
(Zhongguo Zhong Xi Yi Jie He Za Zhi)
Vol. 27
Issue 1
Pg. 60-4
(Jan 2007)
ISSN: 1003-5370 [Print] China |
PMID | 17302067
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antineoplastic Agents, Phytogenic
- Cancer Vaccines
- Membrane Glycoproteins
- PMEL protein, human
- Pmel protein, mouse
- gp100 Melanoma Antigen
- Lentinan
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Topics |
- Adjuvants, Immunologic
(pharmacology, therapeutic use)
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Cancer Vaccines
(administration & dosage, immunology)
- Cell Line
- Cell Line, Tumor
- Combined Modality Therapy
- Dendritic Cells
(cytology, immunology)
- Female
- Humans
- Lentinan
(pharmacology, therapeutic use)
- Melanoma, Experimental
(immunology, pathology, therapy)
- Membrane Glycoproteins
(genetics, immunology)
- Mice
- Mice, Inbred C57BL
- Random Allocation
- Shiitake Mushrooms
(chemistry)
- Survival Analysis
- Transfection
- gp100 Melanoma Antigen
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