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[Study on effect of lentinan in enhancing anti-tumor action of dendritic cytoma vaccine and its mechanism].

AbstractOBJECTIVE:
To improve the anti-tumor effect of dendritic cytoma vaccine (DCV) for finding an effective anti-tumor biotherapy.
METHODS:
DC vaccine prepared by transfection of adenovirus mediated melanoma-associated antigen gene (gp100) into bone marrow-derived dendritic cell (DC) was used to study the immuno-therapeutic effect and the mechanism of lentinan (LNT) in different dosages, used alone or combined with gp100-DC for treatment of B16 melanoma bearing mice.
RESULTS:
After being treated with LNT combining gp100-DC, the growth of malignant melanoma was inhibited with the tumor-free survival in the experimental animals being 66.7%. The treatment could also significantly enhance the activity of cytotoxicity T lymphocyte (CTL) and natural killer (NK) cells, elevate the levels of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in splenocytes, and histological examination showed that a large amount of inflammatory cells infiltrated inside and around the tumor, and obvious necrosis of tumor cells was found.
CONCLUSION:
By combined use with LNT the anti-tumor immuno-reaction of DCV vaccine could be enhanced effectively.
AuthorsJun Wang, Zhi-dong Zhou, Da-jing Xia
JournalZhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 27 Issue 1 Pg. 60-4 (Jan 2007) ISSN: 1003-5370 [Print] China
PMID17302067 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Antineoplastic Agents, Phytogenic
  • Cancer Vaccines
  • Membrane Glycoproteins
  • PMEL protein, human
  • Pmel protein, mouse
  • gp100 Melanoma Antigen
  • Lentinan
Topics
  • Adjuvants, Immunologic (pharmacology, therapeutic use)
  • Animals
  • Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use)
  • Cancer Vaccines (administration & dosage, immunology)
  • Cell Line
  • Cell Line, Tumor
  • Combined Modality Therapy
  • Dendritic Cells (cytology, immunology)
  • Female
  • Humans
  • Lentinan (pharmacology, therapeutic use)
  • Melanoma, Experimental (immunology, pathology, therapy)
  • Membrane Glycoproteins (genetics, immunology)
  • Mice
  • Mice, Inbred C57BL
  • Random Allocation
  • Shiitake Mushrooms (chemistry)
  • Survival Analysis
  • Transfection
  • gp100 Melanoma Antigen

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