Abstract | BACKGROUND: AIMS: To determine vitreous PEDF and total antioxidant levels in patients with proliferative diabetic retinopathy (PDR), and to investigate the relationship between them. METHODS: Vitreous levels of PEDF and total antioxidant capacity were measured by an ELISA in 39 eyes of 36 patients with diabetes and PDR and in 29 eyes of 29 controls without diabetes. RESULTS: Vitreous levels of total antioxidant capacity were significantly lower in patients with diabetes and PDR than in controls (mean (SD) 0.16 (0.05) vs 0.24 (0.09) mmol/l, respectively, p<0.001). PEDF levels correlated positively with total antioxidant status in the vitreous of patients with PDR (r = 0.37, p<0.05) and in controls (r = 0.41, p<0.05). Further, vitreous levels of PEDF in patients with PDR without vitreous haemorrhage (VH(-)) were significantly (p<0.05) decreased, compared with those in the controls or in patients with PDR with vitreous haemorrhage (VH(+); PDR VH(-), 4.5 (1.1) microg/ml; control, 7.4 (4.1) microg/ml; PDR VH(+) 8.5 (3.6) microg/ml). CONCLUSION: This study demonstrates that PEDF levels are associated with total antioxidant capacity of vitreous fluid in humans, and suggests that PEDF may act as an endogenous antioxidant in the eye and could play a protective role against PDR.
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Authors | Masahiko Yokoi, Sho-ichi Yamagishi, Akari Saito, Yumiko Yoshida, Takanori Matsui, Wataru Saito, Shigeki Hirose, Kazuhiro Ohgami, Manabu Kase, Shigeaki Ohno |
Journal | The British journal of ophthalmology
(Br J Ophthalmol)
Vol. 91
Issue 7
Pg. 885-7
(Jul 2007)
ISSN: 0007-1161 [Print] England |
PMID | 17301120
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Eye Proteins
- Nerve Growth Factors
- Serpins
- pigment epithelium-derived factor
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Topics |
- Adult
- Aged
- Antioxidants
(analysis)
- Diabetic Retinopathy
(metabolism)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Eye Proteins
(analysis)
- Female
- Humans
- Male
- Middle Aged
- Nerve Growth Factors
(analysis)
- Oxidation-Reduction
- Serpins
(analysis)
- Vitreous Body
(chemistry)
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