| Abstract | NF-IL6 was originally identified as a DNA binding protein regulating interleukin-1 (IL-1)-stimulated IL-6 expression. Direct cloning of NF-IL6 showed its homology with C/EBP, a hepatocyte- and adipocyte-specific transcription factor. This study showed that the expression of NF-IL6 messenger RNA (mRNA) increased markedly during the differentiation to a (mRNA) increased markedly during the differentiation to a macrophage lineage in mouse myeloid leukemia cells M1, human histiocytic leukemia cells U937, promyelocytic leukemia cells HL-60, and human peripheral monocytes. Particularly in HL-60 cells that undergo granulocyte or macrophage differentiation depending on inducers, NF-IL6 mRNA was specifically upregulated during macrophage differentiation but not granulocyte differentiation. It was also shown that the functional NF-IL6 protein increased during the differentiation of U937 cells. Furthermore, recombinant NF-IL6 was found to bind to the regulatory regions of the IL-1, tumor necrosis factor, granulocyte colony-stimulating factor, and lysozyme genes, which are expressed in mature macrophages. These results suggest that NF-IL6 may possibly be involved as an important transcription factor in the process of activation and/or differentiation of macrophages. |
| Authors | S Natsuka, S Akira, Y Nishio, S Hashimoto, T Sugita, H Isshiki, T Kishimoto
(Affiliation: Institute for Molecular and Cellular Biology, Osaka University, Japan.)
|
| Journal | Blood
(Blood)
Vol. 79
Issue 2
Pg. 460-6
(Jan 15 1992)
ISSN: 0006-4971 UNITED STATES |
| PMID | 1730090
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- CCAAT-Enhancer-Binding Proteins
- DNA-Binding Proteins
- Interleukin-6
- Nuclear Proteins
- RNA, Messenger
- Recombinant Proteins
- Transcription Factors
- DNA
|
| Topics |
- Animals
- Base Sequence
- Binding Sites
- Blotting, Western
- CCAAT-Enhancer-Binding Proteins
- Cell Differentiation
- Cloning, Molecular
- DNA
(metabolism)
- DNA-Binding Proteins
(genetics, metabolism)
- Gene Expression
- Humans
- Interleukin-6
(genetics, pharmacology)
- Leukemia, Monocytic, Acute
- Macrophages
(cytology, metabolism)
- Mice
- Molecular Sequence Data
- Nuclear Proteins
(genetics, metabolism)
- RNA, Messenger
(biosynthesis)
- Recombinant Proteins
(metabolism)
- Regulatory Sequences, Nucleic Acid
- Sequence Homology, Nucleic Acid
- Transcription Factors
(genetics, metabolism)
- Tumor Cells, Cultured
|
