Immune regulatory functions of human beta-defensin-2 in odontoblast-like cells.

Abstract

AIM:

To investigate the effects of human beta-defensins on the expression of genes involved in the host immune response of the dental pulp.

METHODOLOGY:

Human odontoblast-like cells were cultured in Dulbecco's modified Eagle's medium. Cells were stimulated by recombinant human beta-defensins (rhBDs) up to 4 h. RNA was extracted followed by cDNA synthesis (oligo-(dT)-primer). Samples were analysed by real-time polymerase chain reaction (PCR) technology. Genes of interest were: human beta-defensin-1, -2, interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, cyclooxygenase-2, leukotriene-A4-hydrolase, cytosolic phospholipase-A-2 (cPLA(2)), and dentine sialophosphoprotein. Gene expression of beta-actin served as internal standard for normalizing real-time PCR data. Two-way anova and the paired t-test were applied for comparison of the gene expression.

RESULTS:

In odontoblast-like cells rhBD-2 stimulation led to a down-regulation of the gene expression of hBD-1 (P < 0.05), whilst the mRNA expression of IL-6 (P < 0.05), IL-8 (P < 0.05) and cPLA(2) was increased in response to rhBD-2.

CONCLUSION:

The results of the present study suggest immune regulatory functions of human beta-defensin-2 in odontoblast-like cells.

• Authors: H Dommisch, J Winter, C Willebrand, J Eberhard, S Jepsen
• Journal: International endodontic journal (Int Endod J) Vol. 40 Issue 4 Pg. 300-7 (Apr 2007) ISSN: 0143-2885 [Print] England
• PMID: 17298411 (Publication Type: Journal Article)

Chemical References

• DEFB1 protein, human
• DEFB4A protein, human
• DNA, Complementary
• Interleukin-6
• Interleukin-8
• Recombinant Proteins
• beta-Defensins
• Phospholipases A
• Group IV Phospholipases A2
• PLA2G4A protein, human

Topics

• Adult
• Analysis of Variance
• Cells, Cultured
• DNA, Complementary (analysis)
• Dental Pulp (cytology, immunology)
• Gene Expression Regulation
• Group IV Phospholipases A2
• Humans
• Immunity, Innate (genetics)
• Interleukin-6 (biosynthesis, genetics, immunology)
• Interleukin-8 (biosynthesis, genetics, immunology)
• Odontoblasts (immunology)
• Phospholipases A (biosynthesis, genetics, immunology)
• Polymerase Chain Reaction
• Recombinant Proteins (pharmacology)
• beta-Defensins (biosynthesis, genetics, immunology)