IFN-gamma enhances the expression of cell surface receptors for monoclonal nonspecific suppressor factor.

Abstract

Monoclonal nonspecific suppressor factor (MNSF) is a lymphokine derived from a murine T cell hybridoma. The action of MNSF is mediated by specific cell-surface receptors. Since IFN-gamma alters the cellular response to MNSF (M. Nakamura, H. Ogawa, and T. Tsunematsu, J. Immunol. 138, 1799, 1987), we investigated whether IFN-gamma has an effect on the expression of MNSF receptor on target cells. IFN-gamma enhanced the expression of MNSF receptor on both MOPC-31C cells (a murine plasmacytoma line) and EL4 (a murine T lymphoma line). Incubation with IFN-gamma increased the number of specific MNSF-binding sites by about 50 to 90%, with no significant change in binding affinity. IFN-alpha and IFN-beta also increased MNSF binding, although the effect of the saturating amounts was lower than that seen with IFN-gamma. Maximal enhancement of receptor expression was observed after about 15 hr of incubation with IFN-gamma. No demonstrable change occurred in the kinetics of internalization of 125I-MNSF bound to MOPC-31C cells preincubated without or with IFN-gamma.

• Authors: M Nakamura, H Ogawa, T Tsunematsu
• Journal: Cellular immunology (Cell Immunol) Vol. 139 Issue 1 Pg. 131-8 (Jan 1992) ISSN: 0008-8749 [Print] Netherlands
• PMID: 1728961 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interferon-alpha
• Receptors, Cytokine
• Receptors, Immunologic
• Recombinant Proteins
• Suppressor Factors, Immunologic
• monoclonal-nonspecific suppressor factor receptor
• Interferon-beta
• Interferon-gamma

Topics

• Animals
• Endocytosis
• In Vitro Techniques
• Interferon-alpha (pharmacology)
• Interferon-beta (pharmacology)
• Interferon-gamma (pharmacology)
• Mice
• Receptors, Cytokine
• Receptors, Immunologic (physiology)
• Recombinant Proteins
• Suppressor Factors, Immunologic (physiology)
• Time Factors
• Tumor Cells, Cultured