| Abstract | OBJECTIVE: To observe the effect of tetrandrane on activity of collagenase derived from human hypertrophic scar for the sake of clarifying the mechanism as tetrandrane acting on scar. METHODS: The experimental concentration was controled below that of cellproliferation inhibited, SDS-PAGE electrophoresis was adopted to separate collagenase from extracellular matrix, and then activated by trypsin analyzed the activity of collagenase with density scaning apparatus. At the same time quantity of extracellular collagen was measured using improved chloraseptine T oxidizing assay, moreover analyzed correlation between activity of collagenase and quantity of extracellular collagen. RESULTS: In the concentration below the lever of inhibiting fibroblast proliferation, the total activity of collagenase could be significantly increased by tetrandrane with dosage-dependence associated with quantity of extracellular collagen reduced, which was much greater than that of triamcinolone. CONCLUSION: Increasing activity of collagenase on degradation of collagen even in a lower concentration was one of the mechanisms of tetrandrane treating hypertrophic scar. |
| Authors | Zhi-dong Cao, Chong-rong Shi, Chong-ben Huang, Bang-chun Li, Jing-yue Gou, Yuan-lu Liu, Kui Zhang
(Affiliation: Department of Orthopedics, the First Aid Center of Chongqing, Chongqing 400014, China.)
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| Journal | Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
(Zhonghua Zheng Xing Wai Ke Za Zhi)
Vol. 22
Issue 6
Pg. 448-50
(Nov 2006)
ISSN: 1009-4598 China |
| PMID | 17286006
(Publication Type: English Abstract, Journal Article)
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