HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mechanisms of dietary Cu uptake in freshwater rainbow trout: evidence for Na-assisted Cu transport and a specific metal carrier in the intestine.

Abstract
Copper (Cu) is both a vital nutrient and a potent toxicant. The objective of this study was to analyze the mechanistic nature of intestinal Cu transport in rainbow trout using radiolabeled Cu (64Cu) and an in vitro gut sac technique. Reduction of mucosal NaCl levels inhibited Cu transport while increase caused stimulation; Na(2)SO(4) had an identical effect, implicating Na(+) rather than the anion. These responses were unrelated to solvent drag, osmotic pressure or changes in transepithelial potential. The presence of elevated luminal Ag stimulated Cu and Na(+) uptake. Phenamil caused a partial inhibition of both Cu and Na(+) uptake while hypercapnia stimulated Na(+) and Cu transport. Cu uptake was sensitive to luminal pH and inhibited by a tenfold excess of Fe and Zn. These factors had no effect on Na(+ )uptake. On the basis of these results we propose a novel Na(+)-assisted mechanism of Cu uptake wherein the Na(+) gradient stimulates an increase in the H(+) concentration of the brushborder creating a suitable microenvironment for the effective transport of Cu via either DMT1 or Ctr1.
AuthorsSunita Rao Nadella, Martin Grosell, Chris M Wood
JournalJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology (J Comp Physiol B) Vol. 177 Issue 4 Pg. 433-46 (May 2007) ISSN: 0174-1578 [Print] Germany
PMID17279389 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chlorides
  • Sodium Channel Blockers
  • Sulfates
  • sodium sulfate
  • Carbon Dioxide
  • phenylamil
  • Silver
  • Copper
  • Amiloride
  • Sodium
Topics
  • Amiloride (analogs & derivatives, pharmacology)
  • Animals
  • Carbon Dioxide (metabolism)
  • Chlorides (pharmacokinetics)
  • Copper (pharmacokinetics)
  • Hydrogen-Ion Concentration
  • Hypercapnia (metabolism)
  • Intestinal Absorption (drug effects, physiology)
  • Intestinal Mucosa (metabolism)
  • Microvilli (metabolism)
  • Oncorhynchus mykiss (metabolism)
  • Osmolar Concentration
  • Osmotic Pressure
  • Silver (pharmacology)
  • Sodium (pharmacokinetics)
  • Sodium Channel Blockers (pharmacology)
  • Sulfates (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: