| Abstract | Pasteurella multocida B:2 is responsible for haemorrhagic septicaemia in cattle and buffaloes, causing severe economic losses in the developing countries. In the present study, the ahpA gene of P. multocida B:2 (P52) was cloned, sequenced and compared with the previously reported ahpA gene sequence in P. multocida A:1, which is responsible for its haemolytic phenotype. E. coli DH5a cells were further transformed with recombinant plasmid carrying the ahpA gene from P. multocida B:2 (P52) but SDS-PAGE analysis failed to show the expression of haemolysin protein. Slight haemolysis was albeit observed in horse blood agar plates streaked with recombinant E. coli carrying the ahpA gene. Our study indicates that there is 99.6% similarity and 0.4% divergence between ahpA gene of P. multocida B:2 (P52) and P. multocida A: 1, while membrane topology analysis has predicted that ahpA is an inner membrane protein with two strong hydrophobic regions at the N and C terminals. The presence of significant homology in ahpA sequence in A: 1 and B:2 perhaps suggests a common mechanism of pathogenesis in different species of animals. |
| Authors | S Sudha Rani, V K Chaturvedi, P K Gupta, S Joseph, B C Nair, K D Pandey
(Affiliation: N. P. R. E., Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122 UP, India.)
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| Journal | Acta veterinaria Hungarica
(Acta Vet Hung)
Vol. 54
Issue 4
Pg. 449-53
(Dec 2006)
ISSN: 0236-6290 Hungary |
| PMID | 17278717
(Publication Type: Journal Article)
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| Chemical References |
- AhpA protein, Pasteurella multocida
- Bacterial Proteins
|
| Topics |
- Animals
- Bacterial Proteins
(genetics)
- Buffaloes
- Cattle
- Cloning, Molecular
- Pasteurella Infections
(microbiology, veterinary)
- Pasteurella multocida
(classification, genetics, isolation & purification)
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