Both beta-
adrenergic blockade and
bradycardia may contribute to the
therapeutic effect of beta-blockers in chronic
heart failure (CHF). This study tested the relative importance of
bradycardia by comparing
cilobradine (Cilo), a sinus node inhibitor, with a beta-blocker,
metoprolol (Meto), in an established canine model of CHF. Dogs were chronically instrumented for hemodynamic and left ventricular (LV) volume measurements. CHF was created by daily coronary embolization via a chronically implanted coronary (left anterior descending coronary artery)
catheter. After establishment of CHF, control (n=6), Meto (30 mg/day, n=5), Cilo (low) (1 mg/kg/day, n=5), or Cilo (high) (3 mg/kg/day, n=5) was given orally for 12 weeks. Systemic hemodynamics, echocardiography, and pressure volume analysis were measured at baseline, at CHF, and 3 months
after treatment in an awake state.
Protein levels of cardiac
sarcoplasmic reticulum calcium-ATPase (SERCA2a),
ryanodine receptor (
RyR2), and Na+-Ca2+ exchanger (NCX1) were measured by Western blot.
RyR2 protein kinase A (PKA) phosphorylation was determined by back-phosphorylation. After 12 weeks, Meto and Cilo (high and low) produced similar bradycardic effects, accompanied by a significantly improved LV dP/dt versus control [Meto, 2602+/-70; Cilo (low), 2517+/-45; Cilo (high), 2579+/-78; control, 1922+/-115 mm Hg/s; p<0.05]. Both Meto and Cilo (high) normalized
protein levels of SERCA2a and NCX1 and reversed PKA hyperphosphorylation of
RyR2, in contrast to controls. High-dose
cilobradine effectively produced
bradycardia and improved cardiac function after CHF, comparable with
metoprolol. Restored
protein levels of SERCA2a and improved function of
RyR2 may be important mechanisms associated with
cilobradine therapy.