Characterization of liposomes coated with S-layer proteins from lactobacilli.
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| Abstract |
The stability of liposomes coated with S-layer proteins from Lactobacillus brevis and Lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. The interactions of the different S-layer proteins with positively charged liposomes prepared with soybean lecithin or dipalmitoylphosphatidylcholine were studied by means of the variation of the Z potential at different protein-lipid ratios, showing that both proteins were able to attach in a greater extent to the surface of soybean lecithin liposomes. The capacity of these particles to retain carboxyfluorescein or calcein by exposure to bile salts, pancreatic extract, pH change and after a thermal shock showed that both S-layer proteins increased the stability of the liposomes in the same magnitude. The non-glycosylated protein from L. brevis protects more efficiently the liposomes at pH 7 than those from L. kefir even without treatment with glutaraldehyde.
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| Authors | A Hollmann, L Delfederico, G Glikmann, G De Antoni, L Semorile, E A Disalvo |
| Journal | Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1768 Issue 3 Pg. 393-400 (Mar 2007) ISSN: 0006-3002 [Print] Netherlands |
| PMID | 17276386 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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