| Abstract | A polyelectrolyte complex micelle (PECM)-based delivery system for targeting folate (FOL) receptor overexpressing tumor cells is demonstrated using poly(ethylene glycol) (PEG)-conjugated oligonucleotide (ODN). The tumor targeting property was conferred to the PECM by tethering a folate moiety to the distal end of the PEG segment in an anti-sense green fluorescent protein (GFP) ODN-PEG conjugate. Nanoscale PECMs were spontaneously produced from ionic interactions between the ODN-PEG-FOL conjugate and a cationic lipid, lipofectamine (Lf). When treated with FOL receptor overexpressing cells (KB), the PCEMs caused a significant reduction in GFP expression in a dose-dependent manner. This effect was not observed in FOL receptor deficient cells (A549). The enhanced transfection of ODN-PEG-FOL/Lf PECMs to KB cells was caused by FOL receptor mediated endocytosis. The efficiency of target-specific gene suppression by ODN-PEG-FOL/Lf PECMs was maintained even in the presence of 10% fetal bovine serum in the transfection medium. |
| Authors | Sun Hwa Kim, Ji Hoon Jeong, Hyejung Mok, Soo Hyun Lee, Sung Wan Kim, Tae Gwan Park
(Affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.)
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| Journal | Biotechnology progress
(Biotechnol Prog)
2007 Jan-Feb
Vol. 23
Issue 1
Pg. 232-7
ISSN: 1520-6033 United States |
| PMID | 17269693
(Publication Type: Journal Article)
|
| Chemical References |
- Carrier Proteins
- Cations
- Drug Carriers
- Electrolytes
- Lipids
- Micelles
- Oligonucleotides
- Polyethylene Glycols
- Receptors, Cell Surface
- folate-binding protein
- Folic Acid
|
| Topics |
- Carrier Proteins
(metabolism)
- Cations
- Cell Line, Tumor
- Drug Carriers
(chemistry)
- Drug Delivery Systems
(methods)
- Electrolytes
(chemistry)
- Folic Acid
(metabolism)
- Humans
- KB Cells
- Lipids
(chemistry)
- Materials Testing
- Micelles
- Oligonucleotides
(chemistry)
- Polyethylene Glycols
(chemistry)
- Receptors, Cell Surface
(metabolism)
|
