Trehalose-6-phosphate Phosphatase from Mycobacterium tuberculosis induces humoral and cellular immune responses.

Abstract

Trehalose-6-phosphate phosphatase is an enzyme strictly essential for the growth of mycobacteria. Subcellular fractionation of Mycobacterium tuberculosis and M. bovis bacillus Calmette-Guérin (BCG) located the trehalose-6-phosphate phosphatase in the cell wall and membrane fractions. Trehalose-6-phosphate phosphatase induced an increased Th1-type immune response in mice, characterized by an elevated level of interferon-gamma in antigen-stimulated splenocyte culture and a strong IgG2a antibody response. The trehalose-6-phosphate phosphatase was recognized by the sera of tuberculosis patients and BCG-vaccinated donors. The mycobacterial trehalose-6-phosphate phosphatase is an immunodominant antigen, and it may be a candidate for vaccine development for the control of tuberculosis.

• Authors: Min Zhang, Yanping Yang, Ying Xu, Yaqing Qie, Jiuling Wang, Bingdong Zhu, Qingzhong Wang, Ruiliang Jin, Shengfeng Xu, Honghai Wang
• Journal: FEMS immunology and medical microbiology (FEMS Immunol Med Microbiol) Vol. 49 Issue 1 Pg. 68-74 (Feb 2007) ISSN: 0928-8244 [Print] England
• PMID: 17266712 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BCG Vaccine
• Immunoglobulin G
• trehalose-phosphatase
• Phosphoric Monoester Hydrolases

Topics

• Animals
• Antibody Formation (immunology)
• BCG Vaccine (immunology, pharmacology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunity, Cellular (immunology)
• Immunoglobulin G (biosynthesis, blood)
• Mice
• Mice, Inbred C57BL
• Mycobacterium tuberculosis (enzymology, immunology)
• Phosphoric Monoester Hydrolases (immunology, metabolism, pharmacology)
• Tuberculosis (immunology, microbiology)