| Abstract | This study aimed to determine the effect of intranasal exposure to low doses of Pasteurella multocida B:2 on survival of goats challenged with high doses of the same organism. Eighteen goats were selected and divided into three groups. Goats of group 1 were exposed intranasally twice, with a two-week interval, to 7 x 10(6) cfu/ml of live P. multocida B:2. Goats of group 2 were not exposed to P. multocida B:2 but were kept together with the exposed group 1. Goats of group 3 remained as unexposed controls and were kept separated from the other two groups. Serum samples were collected at weekly intervals to determine the antibody levels. At week 5 post exposure, all goats were challenged subcutaneously with 3.7 x 10(10) cfu/ml of live P. multocida B:2. Following challenge exposure, 8 (67%) goats (4 goats from each of groups 1 and 2) were killed owing to haemorrhagic septicaemia. Four goats were killed peracutely within 48 h post challenge, while the other four goats were killed acutely between 2 and 4 days post challenge. None of the goats of group 3 were killed for haemorrhagic septicaemia. Goats of groups 1 and 2 showed significantly (p < 0.05) higher antibody levels following the first intranasal exposure to P. multocida B:2. However, only group 1 retained the significantly (p < 0.05) high antibody levels following a second intranasal exposure, and remained significantly (p < 0.05) higher than groups 2 and 3 at the time of challenge. P. multocida B:2 was successfully isolated from various organs of goats that were killed between 1 and 4 days post challenge. |
| Authors | M Zamri-Saad, Z A Ernie, M Y Sabri
(Affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang. zamri at vet.upm.edu.my)
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| Journal | Tropical animal health and production
(Trop Anim Health Prod)
2006 Oct-Nov
Vol. 38
Issue 7-8
Pg. 541-6
ISSN: 0049-4747 United States |
| PMID | 17265769
(Publication Type: Journal Article)
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| Chemical References |
- Antibodies, Bacterial
- Bacterial Vaccines
|
| Topics |
- Administration, Intranasal
- Animals
- Antibodies, Bacterial
(blood)
- Bacterial Vaccines
(immunology)
- Colony Count, Microbial
(veterinary)
- Goat Diseases
(microbiology, pathology, prevention & control)
- Goats
- Pasteurella Infections
(microbiology, pathology, prevention & control, veterinary)
- Pasteurella multocida
(immunology, pathogenicity)
- Random Allocation
- Time Factors
|
