Study of M. tuberculosis ES-31 and ES-20 antigen levels in different pathogenic grades of lymph node tuberculosis.

Abstract

OBJECTIVE:

To study tuberculous excretory-secretory (ES) 31 and ES-20 antigens in different pathogenic grades of lymph node tuberculosis (TB).

DESIGN:

The study group included lymph node TB patients showing granuloma with mature epithelioid cells based on cytology findings (strong immune response group, SI) and patients showing no granuloma formation and acellular necrosis (weak immune response group, WI). Sandwich ELISA was performed using affinity purified antibodies against Mycobacterium tuberculosis ES-31 and ES-20 antigens to assay free and immune complexed antigen levels in the serum of these patients.

RESULTS:

Higher levels of immune complexed ES-31 (geometric mean titre [GMT] 848) and ES-20 (GMT 1818) antigens than free ES-31 (GMT 462) and ES-20 (GMT 647) were observed in WI patients. There were higher levels of immune complexed ES-20 antigen levels (GMT 1818) in WI patients than in SI lymph node TB patients; the difference was significant (P < 0.05).

CONCLUSION:

Elevated levels of immune complexed ES-20 antigen in patient's serum may be a useful immunological marker for weak immune response patients in lymph node TB.

• Authors: N Shende, V Upadhye, S Kumar, N Gangane, B C Harinath
• Journal: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease (Int J Tuberc Lung Dis) Vol. 11 Issue 2 Pg. 222-6 (Feb 2007) ISSN: 1027-3719 [Print] France
• PMID: 17263295 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigen-Antibody Complex
• Antigens, Bacterial

Topics

• Antigen-Antibody Complex (immunology)
• Antigens, Bacterial (blood)
• Chromatography, Agarose
• Chromatography, Liquid
• Enzyme-Linked Immunosorbent Assay
• Humans
• Mycobacterium tuberculosis (immunology)
• Tuberculosis, Lymph Node (immunology, pathology)