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Phosphorylation of neuronal nitric oxide synthase at Ser847 in the nucleus intermediolateralis after spinal cord injury in mice.

Abstract
We previously demonstrated that Ca2+/calmodulin (CaM)-dependent protein kinase IIalpha (CaM-KIIalpha) can phosphorylate neuronal nitric oxide synthase (nNOS) at Ser847 and attenuate NOS activity in neuronal cells. In the present study we focused on chronological alteration in levels and cellular location of nNOS, phosphorylated (p)-Ser847-nNOS (NP847), CaM-KII and p-Thr286-CaM-KIIalpha following spinal cord injury (SCI) in mice. Western blot analysis showed nNOS to be significantly phosphorylated at Ser847 from 3 h after SCI, peaking at 24 h and gradually decreasing thereafter, and CaM-KII to be colocalized with nNOS after SCI. Immunohistochemical analysis revealed that SCI causes an increase in both NP847 and p-Thr286-CaM-KIIalpha in the nucleus intermediolateralis. These findings suggest that SCI induces p-Thr286-CaM-KIIalpha, which phosphorylates the nNOS at Ser847 in the nucleus intermediolateralis where NO is thought to play a role as a neurotransmitter in autonomic preganglionic neurons. Thus, the NP847 signaling pathway might be involved in the autonomic failure which occurs immediately after SCI.
AuthorsK Osuka, Y Watanabe, N Usuda, K Atsuzawa, C Aoshima, K Yamauchi, M Takayasu, J Yoshida
JournalNeuroscience (Neuroscience) Vol. 145 Issue 1 Pg. 241-7 (Mar 02 2007) ISSN: 0306-4522 [Print] United States
PMID17258865 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Serine
  • Nitric Oxide Synthase Type I
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Camk2a protein, mouse
Topics
  • Animals
  • Blotting, Western (methods)
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases (metabolism)
  • Disease Models, Animal
  • Female
  • Immunohistochemistry (methods)
  • Mice
  • Mice, Inbred C57BL
  • Neurons (enzymology)
  • Nitric Oxide Synthase Type I (metabolism)
  • Phosphorylation
  • Serine (metabolism)
  • Spinal Cord Injuries (pathology)
  • Substantia Gelatinosa (cytology, enzymology)
  • Time Factors

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