Airway inflammation and bronchial remodelling in toluene diisocyanate-exposed BALB/c mouse model.

Abstract

UNLABELLED:

Toluene diisocyanate (TDI), a highly reactive industrial chemical, is one of the leading causes of occupation-related asthma in industrialized countries. The pathogenesis of TDI-induced asthma, however, remains not fully understood, in part due to lack of appropriate animal models. Twenty five female BALB/c mice (age: 8 weeks) were randomly divided into 5 groups: Ovabumin (OVA); OVA peptide amino acid residues No. 323-339 (Pep); TDI; alum and physiological saline. Mice were intraperitoneally injected with 25 microg OVA or pep absorbed on 300 microg alum, 300 microg alum or saline on days 0, 7 and 14. For the TDI group, mice were sensitized subcutaneously with 20 microl neat TDI on day 0; 20 microl of TDI in olive oil (1:10) on days 7 and 14; on days 21-23. Then each group was challenged intranasally with 20 microl of 1% OVA, 1% Pep, 1% TDI, 10% alum and saline respectively. On day 28, mice were killed under pentothal anesthesia. The results demonstrated that neutrophil-dominant inflammation with a few eosinophil infiltration occurred in the peri-bronchial and peri-vascular regions of the lungs. This was accompanied by hyperplasia/hypertrophy of cells lining the airways and mucus production as shown by HE staining. Positive immunohistochemical MBP staining in parenchyma was also shown. Th2 cytokine IL-4 and IgE production were significant increased 5 days after last challenge while IFN-gamma level was below the detection limit.

CONCLUSION:

the clear elevation of IL-4 and IgE could allow to conclude a possible Th2-like dominated allergic response in TDI-exposed BALB/c mouse model.

• Authors: L-Z Sun, S Elsayed, A M Bronstad, T Van Do, A Irgens, N P Aardal, T B Aasen
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 65 Issue 2 Pg. 118-25 (Feb 2007) ISSN: 0300-9475 [Print] England
• PMID: 17257216 (Publication Type: Journal Article)

Chemical References

• Alum Compounds
• Cytokines
• Toluene 2,4-Diisocyanate
• Interleukin-4
• aluminum sulfate
• Immunoglobulin E
• Ovalbumin

Topics

• Alum Compounds (toxicity)
• Animals
• Asthma (chemically induced, immunology, pathology)
• Bronchi (immunology, pathology)
• Bronchoalveolar Lavage Fluid (chemistry, immunology)
• Cytokines (analysis, blood)
• Disease Models, Animal
• Eosinophils (immunology)
• Female
• Immunoglobulin E (analysis, blood)
• Inflammation (chemically induced, immunology, pathology)
• Interleukin-4 (analysis, blood)
• Mice
• Mice, Inbred BALB C
• Neutrophils (immunology)
• Ovalbumin (administration & dosage, immunology)
• Th2 Cells (immunology)
• Toluene 2,4-Diisocyanate (toxicity)