HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Identification of Acanthamoeba sp. in paraffin-embedded CNS tissue from an HIV+ individual by PCR.

Abstract
The opportunistic pathogens, Acanthamoeba and Balamuthia, are the causative agents of the fatal central nervous system (CNS) infection granulomatous amoebic encephalitis. We report an infection of Acanthamoeba in an HIV+ individual. In the present case, multiple lesions were observed in the skin, brain, lung, liver, and bone. A polymerase chain reaction (PCR) assay specific for Acanthamoeba was positive on tissue from a brain biopsy that had been embedded in paraffin. This report demonstrates the need for the consideration of Acanthamoeba infections in HIV+ individuals with skin lesions and multiple lesions throughout the body with CNS involvement. The results of the present study demonstrate that opportunistic amoebic infections can be diagnosed by PCR from paraffin-embedded biopsy material.
AuthorsRebecca C MacLean, Naiel Hafez, Sarika Tripathi, Clarence G Childress, Nitya R Ghatak, Francine Marciano-Cabral
JournalDiagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 57 Issue 3 Pg. 289-94 (Mar 2007) ISSN: 0732-8893 [Print] United States
PMID17254732 (Publication Type: Case Reports, Journal Article)
Topics
  • AIDS-Related Opportunistic Infections (genetics, parasitology)
  • Acanthamoeba (cytology, genetics, pathogenicity)
  • Adult
  • Animals
  • Brain (parasitology)
  • Central Nervous System Protozoal Infections (genetics, parasitology)
  • HIV Infections (parasitology)
  • Histocytochemistry (methods)
  • Histocytological Preparation Techniques (methods)
  • Humans
  • Male
  • Polymerase Chain Reaction
  • Trophozoites (cytology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password:


Research Interface PRO additionally includes drill-down to evidence, articles by author, export to Excel, FDA Link and mobile subscription:
1 year subscription, $490.00 USD