Abstract | PURPOSE: The authors previously reported a growth-promoting factor, REF-1/ TFPI-2, that is specific to retinal pigment epithelial (RPE) cells. The purpose of this study was to determine the genes and proteins of human RPE cells that are altered by exposure to TFPI-2. METHODS: Human primary RPE cells were cultured with or without TFPI-2. Cell extracts and isolated RNA were subjected to proteomic and transcriptomic analyses, respectively. Proteins were separated by two-dimensional gel electrophoresis followed by gel staining and ion spray tandem mass spectrometry analyses. Transcriptomic analysis was performed using a DNA microarray to detect 27,868 gene expressions. RESULTS: CONCLUSIONS: The mechanisms by which TFPI-2 promotes the proliferation of RPE cells may be associated with augmented c-myc synthesis and the activation of E2F in the retinoblastoma protein (Rb)/E2F pathway at the G1 phase of the RPE cells. Activation of ribosomal protein L11 and the Mdm2 complex of the p53 pathway may be counterbalanced by the hyperproliferative conditions.
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Authors | Masahiko Shibuya, Haru Okamoto, Takehiro Nozawa, Jun Utsumi, Venkat N Reddy, Hirotoshi Echizen, Yasuhiko Tanaka, Takeshi Iwata |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 48
Issue 2
Pg. 516-21
(Feb 2007)
ISSN: 0146-0404 [Print] United States |
PMID | 17251444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CDKN1A protein, human
- Cyclin-Dependent Kinase Inhibitor p21
- E2F3 Transcription Factor
- E2F3 protein, human
- Eye Proteins
- Glycoproteins
- MYC protein, human
- Proteome
- Proto-Oncogene Proteins c-myc
- Retinoblastoma Protein
- tissue-factor-pathway inhibitor 2
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Topics |
- Cell Proliferation
- Cells, Cultured
- Cyclin-Dependent Kinase Inhibitor p21
(metabolism)
- E2F3 Transcription Factor
(metabolism)
- Electrophoresis, Gel, Two-Dimensional
- Eye Proteins
(genetics, metabolism)
- Gene Expression Profiling
- Glycoproteins
(pharmacology)
- Humans
- Oligonucleotide Array Sequence Analysis
- Pigment Epithelium of Eye
(cytology, drug effects, metabolism)
- Proteome
- Proto-Oncogene Proteins c-myc
(metabolism)
- Retinoblastoma Protein
(metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Transcription, Genetic
- Up-Regulation
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