Abstract |
Hyperplasia in the hamster cheek pouch was examined using 4 different hyperplastic agents: oil of turpentine 50% v/v in liquid paraffin; vitamin A palmitate 10% w/v in liquid paraffin; 12-O-tetradeconyl-phorbol-13-acetate 16nM in acetone; and ethylphenylpropiolate 0.04mM in acetone. Acetone, paraffin and untreated control groups were also examined. Cheek pouches were painted 3 times a week for up to 4 weeks with each solution. Samples were removed and prepared for light microscopy 24 hours after 2 weeks of painting and 24 hours, 6, 12 and 18 weeks after 4 weeks of painting. Hyperplasia was produced by application of turpentine, vitamin A and TPA after 2 weeks of application. Further increases in epithelial width occurred after 4 weeks of painting in the turpentine and vitamin A groups but a decrease was seen in the TPA group. Six weeks ( vitamin A and TPA groups) or 12 weeks ( turpentine group) after the completion of treatment the epithelium had a normal histological appearance. No differences between the control or EPP treated cheek pouch mucosa could be detected. Turpentine and vitamin A can be used as models of reversible hyperplasia in the hamster cheek pouch.
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Authors | B H Shearer, M D McMillan, H F Jenkinson |
Journal | Journal de biologie buccale
(J Biol Buccale)
Vol. 19
Issue 4
Pg. 315-8
(Dec 1991)
ISSN: 0301-3952 [Print] France |
PMID | 1724238
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alkynes
- Diterpenes
- Retinyl Esters
- keratohyalin
- Vitamin A
- retinol palmitate
- ethylphenylpropiolate
- Keratins
- Tetradecanoylphorbol Acetate
- Turpentine
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Topics |
- Alkynes
(pharmacology)
- Animals
- Cell Nucleus
(drug effects, ultrastructure)
- Connective Tissue
(drug effects, pathology)
- Cricetinae
- Cytoplasmic Granules
(ultrastructure)
- Diterpenes
- Epithelium
(drug effects, pathology)
- Hyalin
- Hyperplasia
- Keratins
- Male
- Mouth Mucosa
(drug effects, pathology)
- Retinyl Esters
- Tetradecanoylphorbol Acetate
(pharmacology)
- Time Factors
- Turpentine
(pharmacology)
- Vitamin A
(analogs & derivatives, pharmacology)
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