Abstract | BACKGROUND: Helicobacter pylori, especially the cytotoxin-associated antigen A (cagA)-positive strains, plays a crucial role in the development of gastric atrophy and gastric cancer. CagA delivered into gastric epithelial cells combines with src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), possibly leading to atrophy/ cancer. Our previous study found that a single-nucleotide polymorphism (SNP; IMS-JST057927) of the PTPN11 gene encoding SHP-2, was associated with gastric atrophy among H. pylori-seropositive subjects. This study aimed to examine the reproducibility of the association among Japanese residing in a different circumstance. METHODS: The subjects were 918 healthy adult Japanese Brazilians from four different areas in Brazil. Blood was sampled from March to May 2001. The target SNP in intron 3 of PTPN11 was genotyped by polymerase chain reaction with confronting two-pair primers (PCR-CTPP). Gastric atrophy was evaluated with serum pepsinogens (PGs); PG I, less than 70 ng/dl and PG I/II ratio, less than 3. RESULTS: The genotype frequency of PTPN11 was in Hardy-Weinberg equilibrium: 65.5% for G/G, 30.4% for G/A, and 4.1% for A/A. The PTPN11 polymorphism had no significant association with H. pylori seropositivity. Among the H. pylori-seropositive subjects, the odds ratios ( ORs) of gastric atrophy were 0.93 (95% confidence interval [CI], 0.59-1.47) for the G/A genotype and 0.31 (95% CI, 0.10-0.95) for the A/A genotype, compared with the G/G genotype. CONCLUSIONS: The present study reproduced the significant association between the A/A genotype and reduced risk of gastric atrophy among Japanese outside Japan. According to the Japan Single Nucleotide Polymorphisms (JSNP) database (db)SNP data, the G allele is very frequent among Japanese and rare in Caucasians. This fact may partly explain the distribution of gastric atrophy/ cancer in the world.
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Authors | Sayo Kawai, Yasuyuki Goto, Lucy S Ito, Sueli M Oba-Shinjo, Miyuki Uno, Samuel K Shinjo, Suely K N Marie, Yoshiko Ishida, Kazuko Nishio, Mariko Naito, Nobuyuki Hamajima |
Journal | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
(Gastric Cancer)
Vol. 9
Issue 4
Pg. 277-83
( 2006)
ISSN: 1436-3291 [Print] Japan |
PMID | 17235629
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Intracellular Signaling Peptides and Proteins
- Protein Phosphatase 2
- PTPN11 protein, human
- Protein Tyrosine Phosphatase, Non-Receptor Type 11
- Protein Tyrosine Phosphatases
- SH2 Domain-Containing Protein Tyrosine Phosphatases
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Topics |
- Adult
- Aged
- Asian People
(genetics)
- Brazil
- Female
- Gastritis, Atrophic
(genetics, microbiology)
- Genetic Predisposition to Disease
- Genotype
- Helicobacter Infections
(complications)
- Helicobacter pylori
(pathogenicity)
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics)
- Japan
- Male
- Middle Aged
- Polymorphism, Single Nucleotide
- Protein Phosphatase 2
- Protein Tyrosine Phosphatase, Non-Receptor Type 11
- Protein Tyrosine Phosphatases
(genetics)
- SH2 Domain-Containing Protein Tyrosine Phosphatases
- src Homology Domains
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