Abstract |
This study aims to clarify the enhancement effect on chromosomal aberrations via selective energy absorption by Cu atoms in cultured bone cancer cells with non-labelled Cu-pyruvaldehyde-bis(N(4)-methylsemicarbazone) ( Cu-PTSM) and monochromatic X rays. The X rays having the energy of the Cu K-shell absorption edge arising from synchrotron radiation (KEK-PF) was used as radiation source. Cu-PTSM labelled with copper radionuclides has been developed for medical imaging using positron emission tomography (PET). Evidence clearly showed that the absorption of X rays in the Cu K-shell caused this enhancement. After cells were treated with 1000 nM Cu-PTSM, the enhancement ratio of the initial yield of isochromatid breaks caused by CuK-H X-ray irradiation to that by 200 kV(p) X rays was approximately 2.8. About 5.6 times of the remaining isochromatid breaks were observed at 4 h in Cu-PTSM treated samples irradiated with CuK-H X rays comparing to that with 200 kV(p) X rays. In this study, uniqueness in physical property such as Cu atom K-shell ionisation was applied for the enhancement of biological effects in cancer cells.
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Authors | Kaoru Takakura, Satoshi Yaguchi, Yuichi Kanansugi, Katsumi Kobayashi, Ryuichi Okayasu, Yasuhisa Fujibayashi |
Journal | Radiation protection dosimetry
(Radiat Prot Dosimetry)
Vol. 122
Issue 1-4
Pg. 188-94
( 2006)
ISSN: 0144-8420 [Print] England |
PMID | 17234644
(Publication Type: Journal Article)
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Chemical References |
- Copper Radioisotopes
- DNA, Neoplasm
- Organometallic Compounds
- Thiosemicarbazones
- copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex
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Topics |
- Adolescent
- Bone Neoplasms
(pathology, physiopathology)
- Chromosome Aberrations
(radiation effects)
- Chromosomes
(genetics, radiation effects)
- Copper Radioisotopes
- DNA, Neoplasm
(genetics, radiation effects)
- Dose-Response Relationship, Radiation
- Female
- Humans
- Organometallic Compounds
(radiation effects)
- Osteosarcoma
(pathology, physiopathology)
- Radiation Dosage
- Staining and Labeling
(methods)
- Thiosemicarbazones
(radiation effects)
- Tumor Cells, Cultured
- X-Rays
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