Abstract | BACKGROUND: RESULTS: Quantitative immunohistochemical analyses showed that, compared to those of the control subjects (n = 9), colonic biopsies from patients with active inflammatory bowel disease (n = 17) had 3- to 7-fold higher mean counts of cells expressing 5-lipoxygenase (P = 0.03), 5-lipoxygenase-activating protein (P = 0.005), and the leukotriene A(4) hydrolase (P = 0.004), which make up the biosynthetic pathway of the potent neutrophil chemotaxin leukotriene B(4). Immunoexpression of the leukotriene C(4) synthase was unaltered (P > 0.2). The increased representation of leukotriene B(4)-pathway enzymes was associated with higher counts of neutrophils (P = 0.0001), macrophages (P = 0.03), eosinophils (P = 0.0004), CD8(+) T cells (P < 0.001), activated T cells (P < 0.05), and B cells (P < 0.05) but not of mast cells (P > 0.9). These eicosanoid and cellular changes were most marked in the subgroup of patients with ulcerative colitis (n = 9), and were absent in patients with quiescent disease (n = 6). The anomalies in the 5-lipoxygenase pathway were accompanied as expected by more cells immunostaining for cytokine-inducible COX-2 (P = 0.004, n = 17), but this study also revealed a greater number of cells expressing COX-1 in the samples from the patients in the ulcerative colitis subgroup (P = 0.03, n = 9). CONCLUSIONS:
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Authors | James Jupp, Keith Hillier, Daniel H Elliott, David R Fine, Adrian C Bateman, Penny A Johnson, Angelica M Cazaly, John F Penrose, Anthony P Sampson |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 13
Issue 5
Pg. 537-46
(May 2007)
ISSN: 1078-0998 [Print] England |
PMID | 17230539
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Leukotrienes
- Leukotriene B4
- Arachidonate 5-Lipoxygenase
- Cyclooxygenase 1
- Cyclooxygenase 2
- Glutathione Transferase
- Epoxide Hydrolases
- leukotriene-C4 synthase
- leukotriene A4 hydrolase
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Arachidonate 5-Lipoxygenase
(metabolism)
- Biosynthetic Pathways
- Colon
(enzymology, metabolism, pathology)
- Cyclooxygenase 1
(metabolism)
- Cyclooxygenase 2
(metabolism)
- Epoxide Hydrolases
(metabolism)
- Female
- Glutathione Transferase
(metabolism)
- Humans
- Immunohistochemistry
- Inflammatory Bowel Diseases
(enzymology, metabolism, pathology)
- Intestinal Mucosa
(enzymology, metabolism)
- Leukocytes
(pathology)
- Leukotriene B4
(metabolism)
- Leukotrienes
(metabolism)
- Macrophages
(pathology)
- Male
- Mast Cells
(pathology)
- Middle Aged
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