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Anti-idiotypic antibodies mimic bovine viral diarrhea virus antigen.

Abstract
Polyclonal rabbit anti-idiotypic antibodies (anti-ids) against two neutralizing murine monoclonal antibodies (mAbs) specific to a bovine viral diarrhea virus (BVDV) glycoprotein, 53 kDa, were produced, purified, and characterized. Each anti-id inhibited the binding of its respective mAb to BVDV antigen in a competitive ELISA and blocked the immunoprecipitation of the 53 kDa protein by the mAb. The anti-ids also inhibited the virus-neutralizing activity of their homologous mAbs. These results suggest that the anti-ids bear an internal image of a BVDV antigen and mimic neutralizing epitopes on the 53 kDa protein. Treatment of MDBK cells with the anti-ids inhibited BVDV infection, indicating that they block a cellular component, such as a virus receptor, required for virus adsorption or entry. Inhibition of the homologous mAb and lack of inhibition of the heterologous mAb indicate that the anti-ids are specific for the unique antigen-binding sites on the mAbs.
AuthorsW Xue, D J Orten, O Y Abdelmagid, M Rider, F Blecha, H C Minocha
JournalVeterinary microbiology (Vet Microbiol) Vol. 29 Issue 3-4 Pg. 201-12 (Nov 1991) ISSN: 0378-1135 [Print] Netherlands
PMID1722928 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Antigens, Viral
  • Epitopes
Topics
  • Animals
  • Antibodies, Anti-Idiotypic (immunology, isolation & purification)
  • Antibodies, Monoclonal (immunology)
  • Antibody Specificity
  • Antigens, Viral (immunology)
  • Binding, Competitive
  • Cattle
  • Diarrhea Viruses, Bovine Viral (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes (immunology)
  • Precipitin Tests

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