Abstract |
Polyclonal rabbit anti-idiotypic antibodies (anti-ids) against two neutralizing murine monoclonal antibodies (mAbs) specific to a bovine viral diarrhea virus (BVDV) glycoprotein, 53 kDa, were produced, purified, and characterized. Each anti-id inhibited the binding of its respective mAb to BVDV antigen in a competitive ELISA and blocked the immunoprecipitation of the 53 kDa protein by the mAb. The anti-ids also inhibited the virus-neutralizing activity of their homologous mAbs. These results suggest that the anti-ids bear an internal image of a BVDV antigen and mimic neutralizing epitopes on the 53 kDa protein. Treatment of MDBK cells with the anti-ids inhibited BVDV infection, indicating that they block a cellular component, such as a virus receptor, required for virus adsorption or entry. Inhibition of the homologous mAb and lack of inhibition of the heterologous mAb indicate that the anti-ids are specific for the unique antigen-binding sites on the mAbs.
|
Authors | W Xue, D J Orten, O Y Abdelmagid, M Rider, F Blecha, H C Minocha |
Journal | Veterinary microbiology
(Vet Microbiol)
Vol. 29
Issue 3-4
Pg. 201-12
(Nov 1991)
ISSN: 0378-1135 [Print] Netherlands |
PMID | 1722928
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
|
Chemical References |
- Antibodies, Anti-Idiotypic
- Antibodies, Monoclonal
- Antigens, Viral
- Epitopes
|
Topics |
- Animals
- Antibodies, Anti-Idiotypic
(immunology, isolation & purification)
- Antibodies, Monoclonal
(immunology)
- Antibody Specificity
- Antigens, Viral
(immunology)
- Binding, Competitive
- Cattle
- Diarrhea Viruses, Bovine Viral
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Epitopes
(immunology)
- Precipitin Tests
|