Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor.

Abstract	Podoplanin (Aggrus) is a mucin-type sialoglycoprotein that plays a key role in tumor cell-induced platelet aggregation. Podoplanin possesses a platelet aggregation-stimulating (PLAG) domain, and Thr52 in the PLAG domain of human podoplanin is important for its activity. Endogenous or recombinant human podoplanin were purified, and total glycosylation profiles were surveyed by lectin microarray. Analyses of glycopeptides produced by Edman degradation and mass spectrometry revealed that the disialyl-corel (NeuAc alpha2-3Gal beta l-3(NeuAc alpha2-6)GalNAc alpha l-O-Thr) structure was primarily attached to a glycosylation site at residue Thr52. Sialic acid-deficient podoplanin recovered its activity after additional sialylation. These results indicated that the sialylated Corel at Thr52 is critical for podoplanin-induced platelet aggregation.
Authors	Mika Kato Kaneko, Yukinari Kato, Akihiko Kameyama, Hiromi Ito, Atsushi Kuno, Jun Hirabayashi, Tomomi Kubota, Koh Amano, Yasunori Chiba, Yasushi Hasegawa, Isoji Sasagawa, Kazuhiko Mishima, Hisashi Narimatsu
Journal	FEBS letters (FEBS Lett) Vol. 581 Issue 2 Pg. 331-6 (Jan 23 2007) ISSN: 0014-5793 [Print] England
PMID	17222411 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide Lectins Membrane Glycoproteins Organophosphorus Compounds PDPN protein, human Peptide Fragments Polysaccharides Recombinant Proteins Threonine N-Acetylneuraminic Acid
Topics	Amino Acid Sequence Animals Blood Platelets (drug effects) CHO Cells Carbohydrate Sequence Cricetinae Cricetulus Glycosylation Humans Lectins (chemistry) Mass Spectrometry Membrane Glycoproteins (chemistry, isolation & purification, pharmacology) Molecular Sequence Data N-Acetylneuraminic Acid (analysis) Organophosphorus Compounds (chemistry) Peptide Fragments (chemistry) Platelet Aggregation Polysaccharides (analysis, chemistry) Protein Array Analysis Recombinant Proteins (chemistry, isolation & purification, pharmacology) Threonine (chemistry)

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