Abstract | OBJECTIVES: METHODS: We analyzed 89 biopsy-proven patients with chronic HCV infection. Patients received interferon-alpha or interferon-alpha plus ribavirin for 6 months and were classified into three groups at 6 months after the conclusion of antiviral therapy according to their response to antiviral therapy: sustained responders (N = 29), relapsers (N = 12), and nonresponders (N = 48). Insulin resistance and beta-cell function were assessed by the homeostasis model assessment method (HOMA-IR and HOMA-%B, respectively). Hepatic expression of IRS1/2 was evaluated by immunoblotting and immunostaining in 14 sustained responders. RESULTS: In nonresponders and relapsers, there were no significant changes in HOMA-IR and HOMA-%B values after antiviral therapy. On the other hand, in sustained responders, HOMA-IR values significantly decreased to 1.7 +/- 0.8 from 3.1 +/- 1.1 (P < 0.05) after antiviral therapy. Similarly, HOMA-%B values significantly decreased to 90.6 +/- 10.0 from 113.7 +/- 15.3 (P < 0.05). Immunoblotting showed a threefold increase in IRS1/2 expression after clearance of HCV. Immunostaining revealed that greater IRS1/2 expression was seen in hepatocytes. CONCLUSIONS: We showed that clearance of HCV improves insulin resistance, beta-cell function, and hepatic IRS1/2 expression.
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Authors | Takumi Kawaguchi, Tatsuya Ide, Eitaro Taniguchi, Eiichi Hirano, Minoru Itou, Shuji Sumie, Yumiko Nagao, Chikatoshi Yanagimoto, Shinichiro Hanada, Hironori Koga, Michio Sata |
Journal | The American journal of gastroenterology
(Am J Gastroenterol)
Vol. 102
Issue 3
Pg. 570-6
(Mar 2007)
ISSN: 0002-9270 [Print] United States |
PMID | 17222321
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- IRS1 protein, human
- IRS2 protein, human
- Insulin Receptor Substrate Proteins
- Intracellular Signaling Peptides and Proteins
- Phosphoproteins
- RNA, Viral
- Receptor, Insulin
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Topics |
- Biopsy
- Female
- Follow-Up Studies
- Gene Expression
- Hepacivirus
(genetics, isolation & purification)
- Hepatitis C
(metabolism, pathology, virology)
- Humans
- Immunoblotting
- Immunohistochemistry
- Insulin Receptor Substrate Proteins
- Insulin Resistance
(physiology)
- Insulin-Secreting Cells
(metabolism, pathology)
- Intracellular Signaling Peptides and Proteins
- Liver
(metabolism, pathology)
- Male
- Middle Aged
- Phosphoproteins
(biosynthesis)
- Prognosis
- RNA, Viral
(analysis)
- Receptor, Insulin
(biosynthesis)
- Viral Load
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