Influence of N-glycan processing disruption on tyrosinase and melanin synthesis in HM3KO melanoma cells.

Abstract

Tyrosinase, a type I membrane glycoprotein, is synthesized and glycosylated in the endoplasmic reticulum (ER) and Golgi. The enzyme is subsequently transported to melanosomes where it participates in melanogenesis. Previous studies showed that the disruption of early ER N-glycan processing by deoxynojirimycin (DNJ), an inhibitor of alpha-glucosidase, suppresses tyrosinase enzymatic activity and melanogenesis. However, the disruption of late glycan processing, mainly performed by ER and Golgi alpha-1,2-mannosidases, on tyrosinase enzymatic activity and melanogenesis remains to be investigated. Following treatment of HM3KO human melanoma cells with deoxymannojirimycin (DMJ), an inhibitor of alpha-1,2-mannosidase, transport of tyrosinase to the melanosome, enzymatic activity, and melanogenesis were reduced in a dose-dependent manner. However, DMJ did not directly inhibit tyrosinase enzymatic activity and expression. Interestingly, an extract of Streptomyces subrutilus culture medium (ESSCM) containing DMJ and DNJ as the main components inhibited glycosylation and transport of tyrosinase to the melanosome as well as melanin synthesis, but with no negative effects on cell viability. These inhibitory effects of ESSCM were stronger than those of DMJ or DNJ alone. Tyrosinase glycosylation and melanogenesis in HM3KO melanoma cells were more effectively inhibited by DMJ and DNJ combined than DMJ or DNJ alone. Accordingly, we propose that ESSCM is a potential candidate for treating undesirable hyperpigmentation conditions, such as melasma, postinflammatory melanoderma, and solar lentigo.

• Authors: Hyunjung Choi, Soomi Ahn, Huikyoung Chang, Nam Suk Cho, Kyungmi Joo, Byeong Gon Lee, Ihseop Chang, Jae Sung Hwang
• Journal: Experimental dermatology (Exp Dermatol) Vol. 16 Issue 2 Pg. 110-7 (Feb 2007) ISSN: 0906-6705 [Print] Denmark
• PMID: 17222224 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Melanins
• Monophenol Monooxygenase
• Mannosidases
• mannosyl-oligosaccharide 1,2-alpha-mannosidase

Topics

• Cell Line
• Glycosylation
• Humans
• Mannosidases (antagonists & inhibitors)
• Melanins (biosynthesis)
• Melanoma (metabolism)
• Melanosomes (metabolism)
• Monophenol Monooxygenase (metabolism)
• Skin Neoplasms (metabolism)
• Streptomyces (chemistry)