Abstract |
Proper chromosome segregation is essential for maintenance of genomic integrity and instability resulting from failure of this process may contribute to cancer. Here, we demonstrate that a mutation in the mitotic regulator separase is responsible for the cell cycle defects seen in the zebrafish mutant, cease&desist (cds). Analysis of cds homozygous mutant embryos reveals high levels of polyploidy and aneuploidy, spindle defects, and a mitotic exit delay. Carcinogenesis studies demonstrated that cds heterozygous adults have a shift in tumor spectrum with an eightfold increase in the percentage of fish bearing epithelial tumors, indicating that separase is a tumor suppressor gene in vertebrates. These data strongly support a conserved cross-species role for mitotic checkpoint genes in genetic stability and epithelial carcinogenesis.
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Authors | Jennifer L Shepard, James F Amatruda, David Finkelstein, James Ziai, K Rose Finley, Howard M Stern, Ken Chiang, Candace Hersey, Bruce Barut, Jennifer L Freeman, Charles Lee, Jonathan N Glickman, Jeffery L Kutok, Jon C Aster, Leonard I Zon |
Journal | Genes & development
(Genes Dev)
Vol. 21
Issue 1
Pg. 55-9
(Jan 01 2007)
ISSN: 0890-9369 [Print] United States |
PMID | 17210788
(Publication Type: Journal Article)
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Chemical References |
- Cell Cycle Proteins
- Endopeptidases
- Separase
- Bromodeoxyuridine
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Topics |
- Animals
- Bromodeoxyuridine
- Carcinoma, Pancreatic Ductal
(etiology, pathology)
- Cell Cycle
- Cell Cycle Proteins
(genetics)
- Disease Susceptibility
- Embryo, Nonmammalian
(cytology, metabolism)
- Endopeptidases
(genetics)
- Genomic Instability
- Heterozygote
- Homozygote
- Intestinal Neoplasms
(etiology, pathology)
- Mitosis
- Mutation
- Neoplasms, Glandular and Epithelial
(etiology, pathology)
- Ploidies
- Separase
- Spindle Apparatus
(genetics, pathology)
- Zebrafish
(genetics, growth & development, metabolism)
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