[Effect of ST6Gal I siRNA-mediated gene silencing on the adhesion and invasion of SW480 cells].

Abstract

AIM:

To study the effect of synthesized ST6Gal I specific siRNA on the adhesion and invasiveness of human colon carcinoma cell line SW480 with over expression of ST6Gal I.

METHODS:

A double strand small interference RNA (siRNA) targeting ST6Gal I was designed and synthesized, and then transfected into SW480 cells by lipofectmine 2000. SW480 cells were cultured and divided into 4 groups: blank control group, liposome control group, non-specific siRNA group and ST6Gal I siRNA group. The expression of ST6Gal I mRNA was examined by RT-PCR and the amount of alpha-2, 6-sialylation on the SW480 cell surface was detected by flow cytometry. The adhesion and invasion of SW480 cells to extracellular matrix (ECM) were analyzed by using CytoMatrix kit and cell invasion assay kit, respectively.

RESULTS:

After SW480 cells were transfected for 48 hours, the expression of ST6Gal I mRNA in ST6Gal I siRNA group was significantly decreased compared with that in the blank control group, liposome control group, and non-specific siRNA group (P<0.05). After SW480 cells were transfected for 72 hours, the amount of alpha-2, 6-sialylation on cell surface, the adhesion and invasion of the cells in ST6Gal I siRNA group were markedly lower than those in the other 3 groups (P<0.05).

CONCLUSION:

The chemically synthesized specific siRNA targeting ST6Gal I can effectively inhibit the expression of ST6Gal I and reduce cell adhesion and invasion to ECM in SW480 cells. Our research is important for further study of anti-tumor treatment with RNA interference.

• Authors: Tian-hong Yuan, Ming-yuan Li, Wan-yi Li, Hong Li, Zhong-hua Jiang
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 23 Issue 1 Pg. 39-41, 45 (Jan 2007) ISSN: 1007-8738 [Print] China
• PMID: 17210104 (Publication Type: English Abstract, Journal Article)

Chemical References

• Lipids
• Lipofectamine
• RNA, Small Interfering
• Sialyltransferases
• beta-D-Galactoside alpha 2-6-Sialyltransferase

Topics

• Cell Adhesion (genetics)
• Cell Line, Tumor
• Colonic Neoplasms (genetics, therapy)
• Flow Cytometry
• Gene Silencing (physiology)
• Humans
• Lipids (genetics)
• Neoplasm Invasiveness (genetics)
• RNA Interference (physiology)
• RNA, Small Interfering (genetics, physiology)
• Reverse Transcriptase Polymerase Chain Reaction
• Sialyltransferases (genetics)
• Transfection
• beta-D-Galactoside alpha 2-6-Sialyltransferase