Abstract |
Peripheral neuropathy is the most frequent neurologic complication associated with human immunodeficiency virus ( HIV) infection, yet its pathogenesis remains poorly understood. To study the mechanisms causing HIV-induced peripheral nervous system disease, we examined trigeminal ganglia obtained from simian immunodeficiency virus (SIV)-inoculated macaques. SIV-infected macaques developed multifocal trigeminal ganglionitis of varying severity characterized by multifocal mononuclear infiltrates, neuronophagia, and neuronal loss resembling reports of HIV-associated changes present in dorsal root ganglia. Neuronal density, measured by calculating the fractional area of trigeminal ganglia occupied by neurons, was significantly lower in SIV-infected macaques versus uninfected macaques (p = 0.001). To characterize the inflammatory cell population and measure productive viral infection in ganglia, trigeminal ganglia from SIV-infected macaques were immunostained for macrophage or cytotoxic lymphocyte markers and for SIV gp41. The extent of macrophage infiltration in trigeminal ganglia was inversely correlated with neuronal loss (p = 0.001), whereas cytotoxic lymphocyte infiltration was not associated with neuronal loss. These studies demonstrate that alterations in the somatosensory ganglia of SIV-infected macaques closely parallel those observed in HIV-infected individuals and show that study of SIV-infected macaques may help elucidate the pathophysiology of HIV-induced peripheral neuropathy.
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Authors | Victoria A Laast, Carlos A Pardo, Patrick M Tarwater, Suzanne E Queen, Todd A Reinhart, Mimi Ghosh, Robert J Adams, M Christine Zink, Joseph L Mankowski |
Journal | Journal of neuropathology and experimental neurology
(J Neuropathol Exp Neurol)
Vol. 66
Issue 1
Pg. 26-34
(Jan 2007)
ISSN: 0022-3069 [Print] England |
PMID | 17204934
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- CD68 antigen, human
- Gene Products, gag
- Viral Regulatory and Accessory Proteins
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Topics |
- Animals
- Antigens, CD
(metabolism)
- Antigens, Differentiation, Myelomonocytic
(metabolism)
- CD4 Lymphocyte Count
(methods)
- Cell Count
(methods)
- Central Nervous System
(pathology)
- Gene Expression Regulation, Viral
(physiology)
- Gene Products, gag
(genetics, metabolism)
- Immunohistochemistry
(methods)
- In Situ Hybridization
(methods)
- Infections
- Macaca
- Microscopy, Confocal
(methods)
- Peripheral Nervous System
(pathology, virology)
- Simian Acquired Immunodeficiency Syndrome
(pathology, virology)
- Simian Immunodeficiency Virus
- Time Factors
- Trigeminal Ganglion
(pathology, virology)
- Viral Load
- Viral Regulatory and Accessory Proteins
(genetics, metabolism)
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