A B cell line from a patient with pure red cell aplasia produces an immunoglobulin that suppresses erythropoiesis.

Abstract

A 4-year-old boy with pure red cell aplasia was investigated. Immunophenotypic analysis of peripheral blood lymphocytes revealed a marked increase of CD20+ cells, which fell from 25.9% in the active stage to 9.7% in remission. The plasma contained a suppressive activity against CFU-e and BFU-e formation by the patient's bone marrow cells, which disappeared when the disease went into remission. Prednisone (2 mg/kg/day) therapy was tried for 5 weeks, but produced no improvement. Subsequently, high-dose gamma-globulin therapy induced complete remission of anemia. A lymphoblastoid B cell line obtained from the patient before therapy produced a factor that suppressed erythropoiesis but not granulopoiesis. The suppressive activity resided in the immunoglobulin fraction and was adsorbed by an anti-immunoglobulin column. These results indicate that expansion of B cells producing an immunoglobulin which suppressed erythropoiesis was involved in the pathogenesis of the disease in this patient.

• Authors: M Nagasawa, H Okawa, J Yata
• Journal: Clinical immunology and immunopathology (Clin Immunol Immunopathol) Vol. 61 Issue 1 Pg. 18-28 (Oct 1991) ISSN: 0090-1229 [Print] United States
• PMID: 1720359 (Publication Type: Case Reports, Journal Article)

Chemical References

• Immunoglobulins
• gamma-Globulins

Topics

• B-Lymphocytes (metabolism)
• Cell Line
• Child, Preschool
• Erythropoiesis (immunology)
• Humans
• Immunoglobulins (biosynthesis, immunology)
• Lymphocyte Subsets (immunology)
• Male
• Phenotype
• Red-Cell Aplasia, Pure (immunology, therapy)
• gamma-Globulins (therapeutic use)