Abstract | CONTEXT: DESIGN: RESULTS: AADC antibodies (Ab) were detected in 12 of 18 (67%) APS I patients and in six of 199 (3%) autoimmune Addison's disease patients. Four patients with autoimmune hepatitis were all positive for AADCAb. None of the 141 healthy control subjects, 82 patients with nonautoimmune adrenal insufficiency, 124 with type 1 diabetes mellitus, and 36 with Graves' disease were found positive. Two epitope regions, corresponding to amino acids 274-299 (E1) and 380-471 (E2) were identified. Localization of E1 was confirmed by displacement studies with synthetic peptides corresponding to peptides of porcine AADC. All 12 AADCAb-positive APS I sera reacted with E1, and seven of 12 (58%) reacted also with E2. E2-specific, but not E1-specific, autoantibodies were associated with a significant inhibition of in vitro AADC enzymatic activity. CONCLUSIONS: We mapped the human AADCAb epitopes to the middle and COOH-terminal regions of the enzyme. Autoantibodies to the COOH-terminal region induce a significant inhibition of enzymatic activity.
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Authors | Paola Candeloro, Carla Borri Voltattorni, Roberto Perniola, Mariarita Bertoldi, Corrado Betterle, Massimo Mannelli, Roberta Giordano, Annamaria De Bellis, Claudio Tiberti, Stefano Laureti, Fausto Santeusanio, Alberto Falorni |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 92
Issue 3
Pg. 1096-105
(Mar 2007)
ISSN: 0021-972X [Print] United States |
PMID | 17200166
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Aromatic-L-Amino-Acid Decarboxylases
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Topics |
- Adolescent
- Adult
- Aged
- Animals
- Aromatic-L-Amino-Acid Decarboxylases
(chemistry, immunology, metabolism)
- Autoantibodies
(blood, chemistry, metabolism)
- Autoimmune Diseases
(blood, immunology)
- Base Sequence
- Child
- Child, Preschool
- Epitope Mapping
- Female
- Humans
- Infant
- Male
- Middle Aged
- Sus scrofa
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