| Abstract | OBJECTIVE: To investigate beta-sitosterol's inhibitory effects on SiHa cells' growth, and the effects on microtubular system in SiHa cell. METHODS: Proliferation inhibition of SiHa cell line was evaluated by MTT assay. Cell cycle of SiHa cells treated with beta-sitosterol was analyzed by flow cytometry. The expression and distribution of microtubule and microtubule associated protein 2 in SiHa cells were investigated by confocal microscopy. Immunoblotting analysis was used to determine tubulin alpha, microtubule associated protein 2, and the proportion of polymerization of tubulin. RESULTS: beta-sitosterol could obviously inhibit the proliferation of SiHa cells, and induce the accumulation of cells in S phase (rather than the G2/M phase) and mitotic arrest in the cell cycle. Confocal microscopy showed an abnormal microtubular network in SiHa cell treated with beta-sitosterol for 5 days, and the expression of microtubule associated protein 2 was marked down-regulated. Further analysis by immunoblotting confirmed the down-regulation of beta-sitosterol on the expression for both microtubule associated protein 2 and tubulin alpha. Moreover, beta-sitosterol reduced the proportion of polymerization of microtubule in a time-dependent manner. CONCLUSION: beta-sitosterol could down-regulate the expression of tubulin alpha and microtubule associated protein 2 in SiHa cells, and inhibit the microtubular polymerization. Our results suggested an anti-microtubule characteristic of beta-sitosterol which might contribute to the proliferation inhibition of SiHa cells. |
| Authors | Li Wang, Yong-jie Yang, Song-hua Chen, Xi-rui Ge, Cong-jian Xu, Sui-qi Gui
(Affiliation: Obstetrics & Gynecology Hospital of Fudan University, Shanghai 200011, China.)
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| Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 86
Issue 39
Pg. 2771-5
(Oct 24 2006)
ISSN: 0376-2491 China |
| PMID | 17199997
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- MAP2 protein, human
- Microtubule-Associated Proteins
- Sitosterols
- Tubulin
- sitosterol
|
| Topics |
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Dose-Response Relationship, Drug
- Female
- Humans
- Immunoblotting
- Microscopy, Confocal
- Microtubule-Associated Proteins
(biosynthesis)
- Microtubules
(drug effects, metabolism)
- Sitosterols
(pharmacology)
- Tubulin
(biosynthesis)
- Uterine Cervical Neoplasms
(metabolism, pathology)
|
