Abstract | AIM: METHODS: Human peritoneal mesothelial cells were cultured from human omentum by an enzyme digestion method, grown in a medium containing 30 mmol/L D-glucose. TGF-beta1 expression and matrix production and turnover were measured in HPMC in the presence and absence of 15 micromol/L troglitazone. The mRNA expressions of TGF-beta(1), Collagen I (Col I) and fibronectin (FN) were determined by semiquantification reverse-transcriptive polymerase chain reaction (RT-PCR). The protein of TGF-beta1 was determined by ELISA and proteins of Col I, FN were determined by western blot. RESULTS: The mRNA expression and protein of TGF-beta(1), Col I, FN were significantly increased in HPMC stimulated with 30 mmol/L D-glucose compared to the control group with F12 media (P < 0.01), which was reversed in the presence of troglitazone (15 micromol/L). Obvious decrease of TGF-beta(1) was found in troglitazone-treated groups as compared to groups stimulated with GS (P < 0.05). Exposure of HPMC to troglitazone reduced collagen I secretion (P < 0.05), and fibronectin secretion (P < 0.05). CONCLUSION:
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Authors | Youming Peng, Hong Liu, Fuyou Liu, Yinghong Liu, Jun Li, Xing Chen |
Journal | Nephrology (Carlton, Vic.)
(Nephrology (Carlton))
Vol. 11
Issue 6
Pg. 516-23
(Dec 2006)
ISSN: 1320-5358 [Print] Australia |
PMID | 17199790
(Publication Type: Journal Article)
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Chemical References |
- Chromans
- Collagen Type I
- Fibronectins
- Hypoglycemic Agents
- PPAR gamma
- RNA, Messenger
- Thiazolidinediones
- Transforming Growth Factor beta1
- Troglitazone
- Glucose
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Topics |
- Blotting, Western
- Cell Division
(drug effects)
- Cell Survival
(drug effects)
- Cells, Cultured
- Chromans
(pharmacology)
- Collagen Type I
(genetics, metabolism)
- Epithelial Cells
(cytology, drug effects, physiology)
- Extracellular Matrix
(drug effects, metabolism)
- Fibronectins
(genetics, metabolism)
- Gene Expression
(drug effects)
- Glucose
(pharmacology)
- Humans
- Hypoglycemic Agents
(pharmacology)
- Immunohistochemistry
- PPAR gamma
(agonists, genetics, metabolism)
- Peritoneum
(cytology)
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Thiazolidinediones
(pharmacology)
- Transforming Growth Factor beta1
(genetics, metabolism)
- Troglitazone
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